Pierce G B, Nakane P K, Martinez-Hernandez A, Ward J M
J Natl Cancer Inst. 1977 May;58(5):1329-45. doi: 10.1093/jnci/58.5.1329.
Two rats with chemically induced transplantable adenocarcinomas of the colon were given pulses of [3H]thymidine, and autoradiography with electron microscopes was used to compare the degrees of differentiation of the stem cells of the tumor and colon. The best differentiated portions of the tumor had acini composed of vacuolated, mucous, and argentaffin cells in various stages of differentiation. Vacuolated and mucous cells incorporated [3H]thymidine and corresponded in degree of differentiation to that of their labeled normal counterparts in the normal colon. An exceedingly undifferentiated labeled cell, hitherto undescribed, was identified in the tumor and crypts of the colon; this may be an undifferentiated colon stem cell that differentiates into vacuolated and mucous stem cells and/or into argentaffin cells. Normal stem cells of the breast and malignant stem cells of spontaneous adenocarcinomas of the breast of C3H mice had comparable degrees of differentiation. Since normal stem cells in these tissues were as undifferentiated as the least differentiated stem cells of the tumors, there is now no need to postulate dedifferentiation as a mechanism to explain the undifferentiated appearance of tumors.
给两只患有化学诱导的可移植性结肠腺癌的大鼠注射[3H]胸腺嘧啶脉冲，并用电子显微镜放射自显影术比较肿瘤和结肠干细胞的分化程度。肿瘤分化最好的部分有腺泡，由处于不同分化阶段的空泡化细胞、黏液细胞和嗜银细胞组成。空泡化细胞和黏液细胞摄取了[3H]胸腺嘧啶，其分化程度与正常结肠中标记的正常对应细胞的分化程度相当。在肿瘤和结肠隐窝中发现了一种迄今未描述的高度未分化的标记细胞；这可能是一种未分化的结肠干细胞，可分化为空泡化和黏液干细胞和/或嗜银细胞。C3H小鼠乳腺的正常干细胞和自发性乳腺腺癌的恶性干细胞具有相当的分化程度。由于这些组织中的正常干细胞与肿瘤中分化程度最低的干细胞一样未分化，因此现在无需假设去分化作为解释肿瘤未分化外观的一种机制。
