与猪CD5反应的单克隆抗体分析：第二届国际猪CD研讨会的结果
Analyses of monoclonal antibodies reacting with porcine CD5: results from the Second International Swine CD Workshop.
作者信息
Pescovitz M D, Book B K, Aasted B, Dominguez J, Bullido R, Trebichavsky I, Novikov B, Valpotic I, Tomaskovic M, Nielsen J, Arn S, Sachs D H, Lunney J K, Boyd P C, Walker J, Lee R, Saalmüller A
机构信息
Indiana University, Dept. of Surgery and Microbiology/Immunology, Indianapolis 46202, USA.
出版信息
Vet Immunol Immunopathol. 1998 Jan 30;60(3-4):269-73. doi: 10.1016/s0165-2427(97)00103-7.
Among the 57 monoclonal antibodies analyzed within the T-cell group, three mAbs fell within cluster T13 including the CD5a standard b53b7 (No. 174). The two new mAbs 1H6/8 (No. 058) and BB6-9G12 (No. 166) both precipitated 55 and 60 kDa proteins that were of similar molecular weights as the standard. Staining patterns on the various cell types were similar. Both new antibodies inhibited the binding of the CD5a reference mAbs b53b7 to peripheral lymphocytes. These mAbs, therefore both react with the CD5a epitope bringing the number of anti-porcine CD5 mAbs to eight, all of which appear to recognize the same epitope.
在T细胞组分析的57种单克隆抗体中，有三种单克隆抗体属于T13簇，包括CD5a标准品b53b7（编号174）。两种新的单克隆抗体1H6/8（编号058）和BB6-9G12（编号166）都沉淀出了分子量与标准品相似的55 kDa和60 kDa蛋白质。在各种细胞类型上的染色模式相似。两种新抗体均抑制CD5a参考单克隆抗体b53b7与外周淋巴细胞的结合。因此，这些单克隆抗体均与CD5a表位发生反应，使抗猪CD5单克隆抗体的数量增至8种，所有这些抗体似乎都识别相同的表位。
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