Lefaucheur J P, Sébille A
Laboratoire de Physiologie, Atelier de Régéneration Neuromusculaire, Faculté de Médecine Saint-Antoine, Paris, France.
Neuromuscul Disord. 1995 Nov;5(6):501-9. doi: 10.1016/0960-8966(95)00012-c.
The cellular events of muscle degeneration and regeneration and their time course were studied in two experimental models of muscle injury mice; (i) the denervation-devascularization (DD) of the extensor digitorum longus (EDL) muscle, which is an ischaemic lesion; (ii) the injection of notexin (NOT), a snake venom, in the tibialis anterior (TA) muscle, resulting in a toxic lesion. Compared to the ischaemic lesion, the toxic lesion was characterized by a more extensive inflammatory infiltrate and a shortened phase of phagocytosis of the damaged myofibres. This allowed the proliferation and differentiation of muscle precursor cells (mpc) to take place earlier and may be further promoted by growth factors released by inflammatory cells. Compared to DD-EDL, NOT-TA showed also a greater conservation of the basement membranes of the necrotic myofibres, that can support the fusion of mpc into myotubes, and a better microvascularization. The onset of muscle regeneration is tightly related to the events which occur during the phase of degeneration.
在两种肌肉损伤小鼠实验模型中研究了肌肉变性和再生的细胞事件及其时间进程；（i）趾长伸肌（EDL）的去神经支配-血管去神经化（DD），这是一种缺血性损伤；（ii）在胫骨前肌（TA）中注射蛇毒诺维毒素（NOT），导致毒性损伤。与缺血性损伤相比，毒性损伤的特征是炎症浸润更广泛，受损肌纤维的吞噬阶段缩短。这使得肌肉前体细胞（mpc）的增殖和分化更早发生，并且可能由炎症细胞释放的生长因子进一步促进。与DD-EDL相比，NOT-TA还显示坏死肌纤维的基底膜保存得更好，这可以支持mpc融合到肌管中，并且微血管化更好。肌肉再生的开始与变性阶段发生的事件密切相关。
