Joseph M D, Kasprzak D J, Crouch S R
Clin Chem. 1977 Jun;23(6):1033-6.
A stopped-flow clinical analyzer is described that makes use of a reaction loop containing immobilized enzyme(s) for the determination of the analyte/substrate. The analyzer has been evaluated by determining glucose with immobilized glucose oxidase. The stopped-flow mixing system was constructed at a current cost of less than $500. The analyzer separates the enzymatic reaction from a followup, spectrophotometric indicator reaction. This separation allows the enzymatic reaction to be used in either a fixed-time, kinetic mode or in an equilibrium mode. Likewise, the indicator reaction can be used in either mode. Results for glucose in blood serum indicate that good precision and accuracy can be obtained.
本文描述了一种停流临床分析仪，它利用含有固定化酶的反应环来测定分析物/底物。该分析仪通过使用固定化葡萄糖氧化酶测定葡萄糖进行了评估。停流混合系统的当前构建成本不到500美元。该分析仪将酶促反应与后续的分光光度指示反应分开。这种分离使得酶促反应可以以固定时间动力学模式或平衡模式使用。同样，指示反应也可以在任何一种模式下使用。血清中葡萄糖的测定结果表明可以获得良好的精密度和准确度。
