一种基于N-乙酰葡糖胺-新糖蛋白诱导精子顶体反应的体外受精新预测测试。

A new predictive test for in-vitro fertilization based on the induction of sperm acrosome reaction by N-acetylglucosamine-neoglycoprotein.

作者信息

Brandelli A, Miranda P V, Añón-Vazquez M G, Marín-Briggiler C I, Sanjurjo C, Gonzalez-Echeverría F, Blaquier J A, Tezón J G

机构信息

Departamento de Bioquimica, Instituto de Biociencias, UFRGS, Porto Alegre, Brazil.

出版信息

Hum Reprod. 1995 Jul;10(7):1751-6. doi: 10.1093/oxfordjournals.humrep.a136168.

DOI:10.1093/oxfordjournals.humrep.a136168

PMID:8582974

Abstract

Neoglycoproteins with N-acetylglucosamine residues (BSA-GlcNAc) induced specifically the acrosome reaction (AR) in human spermatozoa. Our objective was to investigate the relationship between this phenomenon and the invitro fertilization (IVF) rate. Sperm suspensions from IVF protocols were incubated with BSA-GlcNAc (t), using calcium ionophore (i) or medium alone (c) as positive or negative controls. When the normalized AR percentage ratio (STIM) (% ARt-%ARc):(%ARi-%ARc) was compared with fertilization rate in 31 couples from our IVF programme, a positive correlation was found (r = 0.46, P < 0.01). The fertilization rate in patients with STIM > or = 0.2 was higher than in non-responders (STIM < 0.2); 72 +/- 7% compared with 5 +/- 3%. The overall predictive value of this test for adequate fertilization rate (> 30%) was 87%, sensitivity 91% and specificity 78%. False positives were 9% and false negatives 22%. For successful fertilization rates (> 60%), the results were: overall predictive value, 84%; sensitivity 100%; specificity 64%. False positives were 23% and no false negatives were found. The results indicated that the induction of AR in human spermatozoa by GlcNAc-neoglycoproteins could be used to predict their fertilizing ability in vitro.

摘要

带有N - 乙酰葡糖胺残基的新糖蛋白（牛血清白蛋白 - N - 乙酰葡糖胺，BSA - GlcNAc）可特异性诱导人类精子发生顶体反应（AR）。我们的目的是研究这一现象与体外受精（IVF）率之间的关系。将IVF方案中的精子悬液与BSA - GlcNAc（t）一起孵育，使用钙离子载体（i）或单独的培养基（c）作为阳性或阴性对照。当将标准化的顶体反应百分比比率（刺激值，STIM）（（顶体反应t - 顶体反应c）：（顶体反应i - 顶体反应c））与我们IVF项目中31对夫妇的受精率进行比较时，发现存在正相关（r = 0.46，P < 0.01）。刺激值≥0.2的患者的受精率高于无反应者（刺激值<0.2）；分别为72±7%和5±3%。该测试对充足受精率（>30%）的总体预测价值为87%，敏感性为91%，特异性为78%。假阳性为9%，假阴性为22%。对于成功受精率（>60%），结果为：总体预测价值84%；敏感性100%；特异性64%。假阳性为23%，未发现假阴性。结果表明，GlcNAc - 新糖蛋白诱导人类精子发生顶体反应可用于预测其体外受精能力。