Nigro G, Luzi G, Krzysztofiak A, D'Orio F, Aiuti F
Pediatric Institute, La Sapienza University, Rome, Italy.
Pediatr Infect Dis J. 1995 Oct;14(10):891-4. doi: 10.1097/00006454-199510000-00014.
The prevalence of human herpesvirus 6 (HHV-6) infection and the course of human immunodeficiency virus (HIV) disease were investigated in 25 Romanian children with nosocomial HIV-1 infection. HHV-6 IgM and IgG antibodies were detected by enzyme immunoassay (EIA) and immunofluorescence assay (IFA) at the beginning of the study and after 18 months, concomitantly with collection of virologic, immunologic and clinical data. The initial HHV-6 seropositivity was 92% by EIA and 76% by IFA, whereas final testing showed 100% positivity by EIA and 84% by IFA. Positive HHV-6 IgM antibodies were detected in 10 children (40%) by EIA and IFA. Of these 9 children (36%) by EIA and 6 (24%) by IFA had both initial and final IgM antibodies. Children with HHV-6 IgM antibodies had a higher prevalence of pneumonitis than those without (100% vs. 53.3%; P < 0.01). In addition they more frequently showed positive p24 antigen detection (67% vs. 40%) and positive HIV-1 culture (80% vs. 69%). Nevertheless the patients with HHV-6 IgM antibodies showed a slight increase in the final mean CD4+ T cell count (from 1.140 to 1.185 x 10(6)/liter), whereas those with HHV-6 IgG alone showed a statistically significant (P = 0.01) decrease (from 1.395 to 968 CD4+ T-cells x 10(6)/liter). Therefore current or recent HHV-6 infection, as revealed by positive HHV-6 IgM antibodies, appeared to be associated with the development of pneumonitis but not with progression of HIV disease. A possible competitive inhibition of HIV-1 by HHV-6 or a stimulating effect of HHV-6 on CD4+ T-cell production may be suggested.
对25名患有医院获得性HIV-1感染的罗马尼亚儿童进行了人类疱疹病毒6型（HHV-6）感染率及人类免疫缺陷病毒（HIV）疾病病程的调查。在研究开始时及18个月后，采用酶免疫测定法（EIA）和免疫荧光测定法（IFA）检测HHV-6 IgM和IgG抗体，同时收集病毒学、免疫学和临床数据。EIA检测初始HHV-6血清阳性率为92%，IFA为76%；而最终检测EIA显示阳性率为100%，IFA为84%。通过EIA和IFA在10名儿童（40%）中检测到阳性HHV-6 IgM抗体。其中，EIA检测9名儿童（36%）、IFA检测6名儿童（24%）初始和最终均有IgM抗体。有HHV-6 IgM抗体的儿童肺炎患病率高于无该抗体者（100%对53.3%；P<=0.01）。此外，他们更频繁地检测到p24抗原阳性（67%对40%）和HIV-1培养阳性（80%对69%）。然而，有HHV-6 IgM抗体的患者最终平均CD4+T细胞计数略有增加（从1.140升至1.185×10⁶/升），而仅患有HHV-6 IgG的患者则出现统计学显著下降（P=0.01）（从1.395降至968个CD4+T细胞×10⁶/升）。因此，HHV-6 IgM抗体阳性所揭示的当前或近期HHV-6感染似乎与肺炎的发生有关，但与HIV疾病进展无关。可能提示HHV-6对HIV-1有竞争性抑制作用或对CD4+T细胞产生有刺激作用。
