Lyall E G
Department of Haematology and Oncology, Royal Hospital for Sick Children, Edinburgh, United Kingdom.
J Med Virol. 1994 Aug;43(4):373-9. doi: 10.1002/jmv.1890430410.
The serological response of paediatric oncology patients to human herpesvirus-6 (HHV-6) was investigated at presentation and during treatment. Sera from 66 patients presenting with malignancy and 66 controls were examined for anti-HHV-6 IgG by indirect immunofluorescence test (IFA) and enzyme linked immunosorbent assay (ELISA), and for anti-HHV-6 IgM by IFA. Serial samples from 45 children on chemotherapy were examined for anti-HHV-6 IgG by ELISA and sera from selected patients on chemotherapy were examined for IgM by IFA. The response of these patients to four other herpesviruses was also investigated. Ninety percent of presenting patients and controls were IgG positive for HHV-6 by IFA and ELISA. Anti-HHV-6 IgG as measured by the HHV-6 ELISA index declined over time in patients on chemotherapy. Two presenting controls and one leukaemic child with a primary cytomegalovirus seroconversion were anti-HHV-6 IgM positive. In the patient group seropositivity to herpesviruses (types 1-6) increased with age, the mean age of those with IgG to HHV-6 alone was 3.7 years compared to 6.8 years for those with antibodies to all five viruses. At the time of presentation paediatric oncology patients have a similar serological response to HHV-6 as age-matched controls and this IgG response wanes with treatment. Whether this is significant in terms of viral pathogenicity is not known and will require investigation of viral activity in these patients.
对儿科肿瘤患者在初诊时及治疗期间针对人疱疹病毒6型（HHV - 6）的血清学反应进行了研究。通过间接免疫荧光试验（IFA）和酶联免疫吸附测定（ELISA）检测了66例患有恶性肿瘤的患者及66例对照的血清中抗HHV - 6 IgG，并通过IFA检测抗HHV - 6 IgM。通过ELISA检测了45例接受化疗儿童的系列样本中的抗HHV - 6 IgG，并通过IFA检测了部分接受化疗患者的血清中的IgM。还研究了这些患者对其他四种疱疹病毒的反应。通过IFA和ELISA检测，90%的初诊患者及对照的HHV - 6 IgG呈阳性。接受化疗的患者中，通过HHV - 6 ELISA指数测定的抗HHV - 6 IgG随时间下降。两名初诊对照及一名原发性巨细胞病毒血清转化的白血病儿童抗HHV - 6 IgM呈阳性。在患者组中，对疱疹病毒（1 - 6型）的血清阳性率随年龄增加，仅对HHV - 6 IgG阳性者的平均年龄为3.7岁，而对所有五种病毒均有抗体者的平均年龄为6.8岁。初诊时，儿科肿瘤患者对HHV - 6的血清学反应与年龄匹配的对照相似，且这种IgG反应在治疗过程中减弱。这在病毒致病性方面是否具有重要意义尚不清楚，需要对这些患者的病毒活性进行研究。
