Fiocchi A, Restani P, Riva E, Restelli A R, Biasucci G, Galli C L, Giovannini M
5th Pediatric Department, Institute of Pharmacological Sciences, University of Milan, Italy.
J Am Coll Nutr. 1995 Jun;14(3):245-50. doi: 10.1080/07315724.1995.10718503.
This study was designed to evaluate whether peptic treatment of BSA and OSA affects their allergenicity and to evaluate the effect of technological treatments of meat for infant feeding on the allergenicity of these proteins.
Twelve children (8 males and 14 females) suffering from atopic dermatitis (AD), aged 12 months to 4.33 years (mean age 2.21 +/- 1.05 years).
Children suffering from atopic dermatitis (AD), (AD), clinically reactive and SPT-positive to beef, underwent skin prick testing (SPT) and radioallergosorbent test (RAST) with BSA and OSA, before and after peptic treatment. They were tested also with commercially available homogenized bovine meat (HBM) and with freeze-dried bovine (FDBM) and ovine (FDOM) meats. Peptic digestion of BSA and OSA was carried out in a Dubnoff's water bath containing 0.05 mg/ml of pepsin. SPT and RAST were performed for 1 mg/ml of BSA and OSA digested 5 minutes, 2 hours and 4 hours with pepsin; homogenized bovine meat, and FDBM and FDOM. SPT results were expressed as diameters (in mm) of the wheal obtained after SPT; a diameter > or = 3 mm was considered as positive. RAST was considered positive for IgE values 5 > UI/ml.
SPT to native BSA and OSA were positive in all patients. Only 2 of the 12 children were SPT-positive to HBM, FDBM, and FDOM. After digestion, SPTs and were positive as follows: for BSA, 4/12 after 5 minutes peptic treatment, 2/12 after 2 hours and 2/12 after 4 hours; for OSA, 3/12 after 5 minutes peptic treatment, none after 2 and 4 hours. None of RASTs was positive after peptic attack.
Both proteolytic digestion and technological treatment reduced the allergenic potential of meat products.
本研究旨在评估胃蛋白酶处理牛血清白蛋白（BSA）和卵清蛋白（OSA）是否会影响其致敏性，并评估用于婴儿喂养的肉类技术处理对这些蛋白质致敏性的影响。
12名患有特应性皮炎（AD）的儿童（8名男性和4名女性），年龄在12个月至4.33岁之间（平均年龄2.21±1.05岁）。
患有特应性皮炎（AD）且对牛肉临床反应性和皮肤点刺试验（SPT）呈阳性的儿童，在胃蛋白酶处理前后，用BSA和OSA进行皮肤点刺试验（SPT）和放射变应原吸附试验（RAST）。他们还接受了市售均质牛肉（HBM）、冻干牛肉（FDBM）和冻干羊肉（FDOM）的检测。BSA和OSA的胃蛋白酶消化在含有0.05mg/ml胃蛋白酶的杜氏水浴中进行。对用胃蛋白酶消化5分钟、2小时和4小时的1mg/ml BSA和OSA、均质牛肉、FDBM和FDOM进行SPT和RAST检测。SPT结果以SPT后获得的风团直径（mm）表示；直径≥3mm被视为阳性。RAST检测IgE值>5 UI/ml被视为阳性。
所有患者对天然BSA和OSA的SPT均为阳性。12名儿童中只有2名对HBM、FDBM和FDOM的SPT呈阳性。消化后，SPT结果如下：对于BSA，胃蛋白酶处理5分钟后4/12呈阳性，2小时后2/12呈阳性，4小时后2/12呈阳性；对于OSA，胃蛋白酶处理5分钟后3/12呈阳性，2小时和4小时后均无阳性。胃蛋白酶攻击后，所有RAST检测均为阴性。
蛋白水解消化和技术处理均降低了肉类产品的致敏潜力。
