Restani P, Fiocchi A, Beretta B, Velonà T, Giovannini M, Galli C L
Laboratory of Toxicology, Institute of Pharmacological Sciences, University of Milan, Italy.
J Am Coll Nutr. 1997 Aug;16(4):383-9. doi: 10.1080/07315724.1997.10718701.
Although relatively infrequent, meat allergy represents a serious problem for children both because it is generally associated with intolerance to other protein sources and because of the suggested role of meat in stimulating the gastrointestinal development during weaning.
With these considerations, the aim of our work was to improve biological-biochemical knowledge of meat allergy.
This study was performed using in vivo skin prick test (SPT) and in vitro (electrophoresis associated with the immunoblotting technique) tests.
Bovine serum albumin (BSA) and actin were the proteins most frequently involved in binding with the circulating IgE. BSA involvement was confirmed by SPT; the high number of positive responses observed with actin in immunoblotting was not confirmed by SPT data. Cross-reactivity between serum albumins from different animal species was demonstrated. Our studies show that in this group of children, the correspondence between the percentage of sequence identity (phylogenetic similarity) and the number of positive responses was surprisingly high.
Although further studies are necessary, the data reported here provide new biochemical data on meat allergy.
尽管肉类过敏相对不常见，但它对儿童来说是个严重问题，这既是因为它通常与对其他蛋白质来源的不耐受有关，也是因为肉类在断奶期间对胃肠道发育有促进作用。
基于这些考虑，我们研究的目的是增进对肉类过敏的生物化学知识。
本研究采用了体内皮肤点刺试验（SPT）和体外试验（与免疫印迹技术相关的电泳）。
牛血清白蛋白（BSA）和肌动蛋白是最常与循环IgE结合的蛋白质。SPT证实了BSA的参与；免疫印迹中肌动蛋白出现的大量阳性反应未得到SPT数据的证实。不同动物物种血清白蛋白之间的交叉反应性得到了证实。我们的研究表明，在这组儿童中，序列同一性百分比（系统发育相似性）与阳性反应数量之间的对应关系出奇地高。
尽管还需要进一步研究，但此处报告的数据提供了有关肉类过敏的新生物化学数据。
