Ludert J E, Abdul-Latiff L, Liprandi A, Liprandi F
Laboratory of Biology of Viruses, Venezuelan Institute for Scientific Research (IVIC), Caracas, Venezuela.
Res Vet Sci. 1995 Nov;59(3):222-5. doi: 10.1016/0034-5288(95)90006-3.
Picobirnaviruses are a novel group of viruses recently found in the faeces of several species of vertebrates. Examination by polyacrylamide gel electrophoresis of rabbit faecal samples collected in one animal facility revealed the viruses in 23 (11 per cent) of 211 samples. Further analysis by electron microscopy and caesium chloride isopycnic centrifugation confirmed the presence of picobirnaviruses in the samples. The oral inoculation of three newly weaned rabbits with purified viruses resulted in the excretion of a virus with an electropherotype similar to the inoculum, by two of the three inoculated animals. Maximal viral shedding was detected 13 days after inoculation. No sign of diarrhoea was observed either in the inoculated animals or in the virus excreting animals surveyed. No antibody activity could be detected in the paired serum samples taken from the inoculated animals.
微小双股RNA病毒是最近在几种脊椎动物粪便中发现的一类新型病毒。在一个动物饲养设施中收集的兔粪便样本经聚丙烯酰胺凝胶电泳检测，在211份样本中的23份（11%）中发现了这种病毒。通过电子显微镜和氯化铯等密度离心进一步分析，证实样本中存在微小双股RNA病毒。用纯化病毒经口接种3只刚断奶的兔子，3只接种动物中的2只排出了一种电泳型与接种物相似的病毒。接种后13天检测到最大病毒排出量。在接种动物或接受调查的病毒排泄动物中均未观察到腹泻迹象。从接种动物采集的配对血清样本中未检测到抗体活性。
