Buzinaro M G, Freitas P P S, Kisiellius J J, Ueda M, Jerez J A
Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Universidade Estadual Paulista, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900 Jaboticabal, Estado de São Paulo, Brazil.
Vet J. 2003 Sep;166(2):185-7. doi: 10.1016/s1090-0233(03)00031-5.
To determine the incidence of rotavirus infection among dairy herds in the State of São Paulo, Brazil, 576 faecal samples obtained from calves aged 1-45 days with and without diarrhoea, reared on 63 dairy cattle farms, were analyzed. Polyacrylamide gel electrophoresis (PAGE) identified 28 samples positive for group A rotavirus, while four samples, two diarrhoeic and two non-diarrhoeic, showed a bisegmented genome with a typical picobirnavirus pattern. Electron microscopy revealed spherical virus particles with a diameter of 37 nm and without a defined surface structure. The present study is the first report of a bisegmented virus identified in cattle in Brazil.
为确定巴西圣保罗州奶牛场中轮状病毒感染的发生率，对从63个奶牛场饲养的1至45日龄有腹泻和无腹泻的犊牛采集的576份粪便样本进行了分析。聚丙烯酰胺凝胶电泳（PAGE）鉴定出28份A组轮状病毒阳性样本，而4份样本（2份腹泻样本和2份非腹泻样本）显示出具有典型微小双节段RNA病毒模式的双节段基因组。电子显微镜显示出直径为37 nm且无明确表面结构的球形病毒颗粒。本研究是巴西首次报道在牛中鉴定出双节段病毒。
