Giordano M O, Martinez L C, Rinaldi D, Gúinard S, Naretto E, Casero R, Yacci M R, Depetris A R, Medeot S I, Nates S V
Instituto de Virología Dr. J.M. Vanella Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.
J Acquir Immune Defic Syndr Hum Retrovirol. 1998 Aug 1;18(4):380-3. doi: 10.1097/00042560-199808010-00010.
Diarrhea due to enteric pathogens is an important complication of advanced HIV infection. Picobirnaviruses are agents recently linked with human enteritis. In total, 197 fecal samples collected from HIV-infected and noninfected patients with and without diarrhea were investigated for the presence of rotavirus and picobirnavirus by polyacrylamide gel electrophoresis. Picobirnavirus was detected in 8.8% of 57 HIV-infected patients with diarrhea, but it was detected in neither those without diarrhea (p<.018) nor in the group of subjects uninfected with HIV (p<.022). All genomic electropherotypes of picobirnavirus strains had a wide pattern. Picobirnavirus genome segments varied in size between 2.4 and 2.7 and 1.6 and 1.9 kbp for the slow and fast migrating bands, respectively. Rotaviruses were not detected in any of the clinical groups studied. Two methods for the extraction of nucleic acid-phenol/chloroform and guanidinium thiocynate (GTC)/silica-were compared. Detection of picobirnavirus by polyacrylamide gel electrophoresis was 2.5 times more sensitive following guanidinium thiocynate RNA extraction. This investigation offers preliminary results about the circulation of picobirnavirus in HIV-infected patients in Córdoba, Argentina.
肠道病原体引起的腹泻是晚期HIV感染的重要并发症。微小双股RNA病毒是最近与人类肠炎相关的病原体。通过聚丙烯酰胺凝胶电泳，对197份分别来自感染和未感染HIV且有或无腹泻症状患者的粪便样本进行检测，以确定是否存在轮状病毒和微小双股RNA病毒。在57名感染HIV且腹泻的患者中，8.8%检测到微小双股RNA病毒，但在未腹泻的患者中未检测到（p<0.018），在未感染HIV的受试者组中也未检测到（p<0.022）。微小双股RNA病毒株的所有基因组电泳型均呈现广泛的图谱。微小双股RNA病毒基因组片段大小各异，慢迁移带在2.4至2.7kbp之间，快迁移带在1.6至1.9kbp之间。在所研究的任何临床组中均未检测到轮状病毒。比较了两种核酸提取方法——酚/氯仿法和异硫氰酸胍（GTC）/硅胶法。采用异硫氰酸胍RNA提取后，通过聚丙烯酰胺凝胶电泳检测微小双股RNA病毒的灵敏度提高了2.5倍。本研究为阿根廷科尔多瓦感染HIV患者中微小双股RNA病毒的传播提供了初步结果。
