Oude Elferink S J, Maas R N, Harmsen H J, Stams A J
Department of Microbiology, Wageningen Agricultural University, The Netherlands.
Arch Microbiol. 1995 Aug;164(2):119-24.
From granular sludge of an upflow anaerobic sludge bed (UASB) reactor treating paper-mill wastewater, a sulfate-reducing bacterium (strain ASRB1) was isolated with acetate as sole carbon and energy source. The bacterium was rod-shaped, (1.4-1.9 x 2.5-3.4 microns), nonmotile, and gram-negative. Optimum growth with acetate occurred around 37 degrees C in freshwater medium (doubling time: 3.5-5.0 days). The bacterium grew on a range of organic acids, such as acetate, propionate, and butyrate, and on alcohols, and grew autotrophically with H2, CO2, and sulfate. Fastest growth occurred with formate, propionate, and ethanol (doubling time: approx. 1.5 days). Strain ASRB1 clusters with the delta subdivision of Proteobacteria and is closely related to Syntrophobacter wolinii, a syntrophic propionate oxidizer. Strain ASRB1 was characterized as a new genus and species: Desulforhabdus amnigenus.
从处理造纸厂废水的上流式厌氧污泥床（UASB）反应器的颗粒污泥中，分离出一株以乙酸盐作为唯一碳源和能源的硫酸盐还原菌（菌株ASRB1）。该菌呈杆状，（1.4 - 1.9×2.5 - 3.4微米），无运动性，革兰氏阴性。在淡水培养基中，以乙酸盐为底物时，最适生长温度约为37℃（倍增时间：3.5 - 5.0天）。该菌能利用多种有机酸，如乙酸盐、丙酸盐和丁酸盐，也能利用醇类，并且能以氢气、二氧化碳和硫酸盐作为自养生长的底物。以甲酸盐、丙酸盐和乙醇为底物时生长最快（倍增时间：约1.5天）。菌株ASRB1属于变形菌门的δ亚群，与共生丙酸盐氧化菌沃氏互营杆菌密切相关。菌株ASRB1被鉴定为一个新的属和种：产氨脱硫杆菌。
