Sang Q A
Department of Chemistry, Florida State University, Tallahasse 32306-3006, USA.
Biochem Mol Biol Int. 1995 Oct;37(3):573-81.
Ceruloplasmin (Cp) is a major copper transporting plasma protein. The susceptibility of porcine Cp by human leukocyte elastase and plasmin has been investigated. The 116 and 48 kDa bands induced by both proteinases have the same N-terminal sequences which are identical to that of the 132 kDa Cp species, KDKHYYIGIVET. The 70 kDa band generated by elastase has a N-terminal sequence of IGGKVVVYYYIA, and the 68 kDa band generated by plasmin has that of TIIEDAIVGKKV. The 19 kDa bands produced by elastase and plasmin have N-terminal sequences of FNPIKNLLFFLL and VFNPIKNLLFFL, respectively. The novel observations suggest that, in addition to plasmin, leukocyte elastase may play a role in angiogenesis and inflammation by digesting Cp.
铜蓝蛋白（Cp）是一种主要的铜转运血浆蛋白。已经研究了人白细胞弹性蛋白酶和纤溶酶对猪铜蓝蛋白的敏感性。两种蛋白酶诱导产生的116 kDa和48 kDa条带具有相同的N端序列，与132 kDa铜蓝蛋白物种的N端序列相同，即KDKHYYIGIVET。弹性蛋白酶产生的70 kDa条带的N端序列为IGGKVVVYYYIA，纤溶酶产生的68 kDa条带的N端序列为TIIEDAIVGKKV。弹性蛋白酶和纤溶酶产生的19 kDa条带的N端序列分别为FNPIKNLLFFLL和VFNPIKNLLFFL。这些新发现表明，除了纤溶酶外，白细胞弹性蛋白酶可能通过消化铜蓝蛋白在血管生成和炎症中发挥作用。
