Yesilsoy C, Whitaker E, Cleveland D, Phillips E, Trope M
Department of Endodontology, Temple University School of Dentistry, Philadelphia, PA 19140, USA.
J Endod. 1995 Oct;21(10):513-5. doi: 10.1016/s0099-2399(06)80524-8.
Three dilutions of the commonly used irrigant sodium hypochlorite (0.5%, 2.5%, and 5.25%) and the potential irrigants Peridex, chlorhexidine gluconate (0.12%), and Therasol were tested. Alcohol and saline served as controls also. The in vitro portion of this study used four different microorganisms (Streptococcus mutans, Peptostreptococcus micros, Prevotella intermedius, and Porphyromonas gingivalis) to determine antimicrobial effect. Presterilized 7-mm diameter Whatman paper disks soaked with the test solution were prepared, and disks were placed onto the previously seeded agar petri dishes. Each dish was incubated aerobically or anaerobically according to the microorganisms used. An in vivo animal model (guinea pig) was used to examine subcutaneous local tissue reactions using the same materials; 0.1 ml of each test solution was injected subcutaneously into predetermined locations on the animal dorsum. Test site histological examination was done 2 h, 2 days, and 2 wk after the injections. The results of this comparative study indicate Peridex (chlorhexidine gluconate) and Therasol may have good potential for endodontic usage.
对常用冲洗剂次氯酸钠的三种稀释液（0.5%、2.5%和5.25%）以及可能的冲洗剂派丽奥、葡萄糖酸氯己定（0.12%）和Therasol进行了测试。酒精和生理盐水也用作对照。本研究的体外部分使用了四种不同的微生物（变形链球菌、微小消化链球菌、中间普氏菌和牙龈卟啉单胞菌）来确定抗菌效果。制备预先灭菌的、浸泡有测试溶液的直径7毫米的沃特曼纸圆盘，并将圆盘放置在预先接种的琼脂培养皿上。根据所使用的微生物，每个培养皿进行需氧或厌氧培养。使用相同的材料，采用体内动物模型（豚鼠）来检查皮下局部组织反应；将0.1毫升每种测试溶液皮下注射到动物背部的预定位置。在注射后2小时、2天和2周进行测试部位的组织学检查。这项比较研究的结果表明，派丽奥（葡萄糖酸氯己定）和Therasol可能具有良好的牙髓治疗应用潜力。
