风疹抗体酶联免疫吸附测定：一种简单的抗原制备方法。初步报告。
Enzyme linked immunosorbent assay for rubella antibodies: a simple method of antigen production. A preliminary report.
作者信息
de Souza V A, Sumita L M, Otsubo M E, Takei K, Pannuti C S
机构信息
Departamento de Doenças Infecciosas e Parasitárias, Faculdade de Medicina da Universidade de São Paulo, Brazil.
出版信息
Rev Inst Med Trop Sao Paulo. 1995 Jul-Aug;37(4):357-9. doi: 10.1590/s0036-46651995000400013.
A simple method of rubella antigen production by treatment with sodium desoxycholate for use in enzyme immunoassay (IMT-ELISA) is presented. When this assay was compared with a commercial test (Enzygnost-Rubella, Behring), in the study of 108 sera and 118 filter paper blood samples, 96.9% (219/226) overall agreement and correlation coefficient of 0.90 between absorbances were observed. Seven samples showed discordant results, negative by the commercial kit and positive by our test. Four of those 7 samples were available, being 3 positive by HI.
本文介绍了一种通过脱氧胆酸钠处理生产风疹抗原的简单方法，用于酶免疫测定（IMT-ELISA）。在对108份血清和118份滤纸血样的研究中，将该测定法与商业检测法（Enzygnost-Rubella，贝林公司）进行比较时，观察到总体一致性为96.9%（219/226），吸光度之间的相关系数为0.90。有7个样本结果不一致，商业试剂盒检测为阴性，而我们的检测为阳性。这7个样本中有4个样本可用，其中3个通过血凝抑制试验（HI）检测为阳性。
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