Hofmann K J, Neeper M P, Markus H Z, Brown D R, Müller M, Jansen K U
Department of Virus and Cell Biology, Merck Research Laboratories, West Point, Pennsylvania 19486, USA.
J Gen Virol. 1996 Mar;77 ( Pt 3):465-8. doi: 10.1099/0022-1317-77-3-465.
The major capsid protein L1 of human papillomaviruses (HPVs) has been identified as a promising candidate antigen for a prophylactic HPV vaccine. Since amino acid sequence heterogeneity has been demonstrated for the L1 genes within individual HPV types, nucleotide sequences of L1 were determined from six HPV-18 clinical isolates and the cervical carcinoma cell line SW756 and compared to the published HPV-18 prototype sequence. The sequences were almost identical between the clinical isolates and SW756 but differed markedly from the published prototype sequence. Resequencing the prototype HPV-18 revealed that these differences were due to sequencing artifacts of the prototype HPV-18 sequence archived in GenBank. Thus, the HPV-18 L1 genes seem to display a very high level of sequence conservation. The HPV-18 L1 gene derived from SW756 was expressed in Saccharomyces cerevisiae and self-assembly of the L1 protein into virus-like particles was demonstrated.
人乳头瘤病毒（HPV）的主要衣壳蛋白L1已被确定为预防性HPV疫苗的一个有前景的候选抗原。由于已证明个体HPV类型内的L1基因存在氨基酸序列异质性，因此从六个HPV-18临床分离株和宫颈癌细胞系SW756中确定了L1的核苷酸序列，并与已发表的HPV-18原型序列进行比较。临床分离株与SW756之间的序列几乎相同，但与已发表的原型序列有明显差异。对原型HPV-18进行重新测序发现，这些差异是由于GenBank中存档的原型HPV-18序列的测序假象所致。因此，HPV-18 L1基因似乎表现出非常高的序列保守性。源自SW756的HPV-18 L1基因在酿酒酵母中表达，并证明L1蛋白可自组装成病毒样颗粒。
