木薯普通花叶病毒及一种缺陷RNA种类的特性分析

Characterization of cassava common mosaic virus and a defective RNA species.

作者信息

Calvert L A, Cuervo M I, Ospina M D, Fauquet C M, Ramirez B C

机构信息

Centro Internacional de Agricultura Tropical (CIAT), Cali, Colombia.

出版信息

J Gen Virol. 1996 Mar;77 ( Pt 3):525-30. doi: 10.1099/0022-1317-77-3-525.

DOI:10.1099/0022-1317-77-3-525

PMID:8601791

Abstract

The genome of cassava common mosaic potexvirus (CsCMV) has been sequenced from cDNA clones and consists of 6376 nucleotides (nt). A 76 nt untranslated region (UTR) at the 5' terminus was followed by ORF1 which potentially encodes a protein of 1449 amino acids (aa). ORFs 2, 3, and 4 were predicted to encode proteins of 231, 112 and 97 aa, respectively. ORF5 potentially encodes a 229 aa protein of 25 kDa that is similar to the coat proteins of other potexviruses. The 3'-terminal UTR of 114 nt was followed by a poly(A) tail. The genomic organization of the CsCMV genome is similar to that of other potexviruses. A cDNA clone that was apparently obtained from a defective RNA species contained both the 5' and 3' UTRs and an ORF that potentially encodes the first 263 aa of ORF1 and the last 33 aa of the coat protein. Defective RNA species were found both in purified preparations of the virus and in total nucleic acid isolated from CsCMV-infected plants.

摘要

木薯普通花叶马铃薯X病毒（CsCMV）的基因组已通过cDNA克隆进行测序，由6376个核苷酸（nt）组成。5'端有一个76 nt的非翻译区（UTR），其后是ORF1，它可能编码一个由1449个氨基酸（aa）组成的蛋白质。预测ORF2、ORF3和ORF4分别编码231、112和97个氨基酸的蛋白质。ORF5可能编码一个25 kDa、由229个氨基酸组成的蛋白质，该蛋白质与其他马铃薯X病毒的外壳蛋白相似。114 nt的3'端UTR后接一个poly(A)尾。CsCMV基因组的组织结构与其他马铃薯X病毒相似。一个显然从缺陷RNA物种获得的cDNA克隆包含5'和3' UTR以及一个ORF，该ORF可能编码ORF1的前263个氨基酸和外壳蛋白的最后33个氨基酸。在病毒的纯化制剂和从CsCMV感染植物中分离的总核酸中均发现了缺陷RNA物种。