Kammerer W, Cove D J
Department of Genetics, University of Leeds, Leeds, U.K.
Mol Gen Genet. 1996 Feb 25;250(3):380-2. doi: 10.1007/BF02174397.
Genetic analysis of the progeny of crosses involving strains of the moss Physcomitrella patens obtained by re-transforming a stable transgenic line, indicates that the plasmid used for re-transformation inserts at or near the chromosomal location of the related plasmid used to obtain the original transgenic line. The resulting structure may be subject to gene silencing.
对通过重新转化稳定转基因系获得的苔藓小立碗藓菌株杂交后代进行的遗传分析表明,用于重新转化的质粒插入到用于获得原始转基因系的相关质粒的染色体位置处或附近。由此产生的结构可能会发生基因沉默。