小立碗藓转基因株系再转化效应的遗传分析

Genetic analysis of the effects of re-transformation of transgenic lines of the moss Physcomitrella patens.

作者信息

Kammerer W, Cove D J

机构信息

Department of Genetics, University of Leeds, Leeds, U.K.

出版信息

Mol Gen Genet. 1996 Feb 25;250(3):380-2. doi: 10.1007/BF02174397.

DOI:10.1007/BF02174397

PMID:8602154

Abstract

Genetic analysis of the progeny of crosses involving strains of the moss Physcomitrella patens obtained by re-transforming a stable transgenic line, indicates that the plasmid used for re-transformation inserts at or near the chromosomal location of the related plasmid used to obtain the original transgenic line. The resulting structure may be subject to gene silencing.

摘要

对通过重新转化稳定转基因系获得的苔藓小立碗藓菌株杂交后代进行的遗传分析表明，用于重新转化的质粒插入到用于获得原始转基因系的相关质粒的染色体位置处或附近。由此产生的结构可能会发生基因沉默。