Kayser H, Meisel H
Institut für Humanernährung und Lebensmittelkunde der Christian-Albrechts-Universität, Kiel, Germany.
FEBS Lett. 1996 Mar 25;383(1-2):18-20. doi: 10.1016/0014-5793(96)00207-4.
The in vitro modulation of the proliferation of human peripheral blood lymphocytes by different synthetic peptides derived from milk proteins was investigated. Therefore, proliferation changes were followed up after incorporation of BrdU into the DNA, and the influence on protein biosynthesis was measured using the [3H]leucine incorporation test. Tyr-Gly and Tyr-Gly-Gly significantly enhanced (maximal 90 and 35%, respectively) the proliferation of PBL. For beta-casomorphin-7 and beta-casomorphin-10,lymphocyte proliferation was suppressed at lower concentrations, but stimulated at higher concentrations (> or = 10(-7) mol/l). Protein synthesis was stimulated (maxima at 25%) only with Tyr-Gly and Tyr-Gly-Gly. The findings point to a need for further studies on the possible function of peptides derived from milk proteins as orally bioavailable immunopotentiatory compounds.
研究了源自乳蛋白的不同合成肽对人外周血淋巴细胞增殖的体外调节作用。因此，在将BrdU掺入DNA后跟踪增殖变化，并使用[3H]亮氨酸掺入试验测量对蛋白质生物合成的影响。Tyr-Gly和Tyr-Gly-Gly显著增强了（分别最大增强90%和35%）外周血淋巴细胞的增殖。对于β-酪蛋白吗啡-7和β-酪蛋白吗啡-10，较低浓度下淋巴细胞增殖受到抑制，但较高浓度（≥10-7mol/l）下受到刺激。仅Tyr-Gly和Tyr-Gly-Gly刺激了蛋白质合成（最大增幅为25%）。这些发现表明需要进一步研究源自乳蛋白的肽作为口服生物可利用免疫增强化合物的可能功能。
