Rana N, Braun D P, House R, Gebel H, Rotman C, Dmowski W P
Institute for the Study and Treatment of Endometriosis, Grant Hospital, Chicago, Illinois 60614, USA.
Fertil Steril. 1996 May;65(5):925-30.
To evaluate basal (constitutive) and stimulated synthesis of tumor necrosis factor alpha (TNF alpha), interleukin (IL)-8, IL-10 by peritoneal macrophages (PM) in women with endometriosis.
Peritoneal macrophages were cultured in the presence or absence of lipopolysaccharide (LPS) for 24 hours. Peritoneal fluids (PF) and PM supernatants were assayed for cytokines using ELISA.
Institute for the Study and Treatment of Endometriosis and university-based research laboratories.
Fertile controls undergoing tubal ligation (n = 8) and women with endometriosis (n = 17).
Peritoneal fluid samples were obtained at the time of diagnostic laparoscopy (endometriosis group) or laparoscopy for tubal ligation; both were performed in the midluteal phase of the cycle.
Both basal and LPS stimulated production of TNF alpha, IL-8, and IL-10 by the PM were elevated significantly in women with endometriosis as compared with the fertile controls.
This study demonstrated that cytokines TNF alpha, IL-8, and IL-10 are synthesized at greater than normal levels by basal and stimulated PM from women with endometriosis. The levels of TNF alpha and IL-8 correlated with the levels in the PF, suggesting that PM are the principal source of these cytokines in the PF.
评估子宫内膜异位症女性腹膜巨噬细胞（PM）中肿瘤坏死因子α（TNFα）、白细胞介素（IL）-8、IL-10的基础（组成性）合成及刺激后合成情况。
腹膜巨噬细胞在有或无脂多糖（LPS）存在的情况下培养24小时。使用酶联免疫吸附测定法（ELISA）检测腹膜液（PF）和PM上清液中的细胞因子。
子宫内膜异位症研究与治疗研究所及大学研究实验室。
接受输卵管结扎的生育期对照者（n = 8）和子宫内膜异位症女性（n = 17）。
在诊断性腹腔镜检查（子宫内膜异位症组）或输卵管结扎腹腔镜检查时获取腹膜液样本；两者均在月经周期的黄体中期进行。
与生育期对照者相比，子宫内膜异位症女性PM中基础及LPS刺激后的TNFα、IL-8和IL-10生成均显著升高。
本研究表明，子宫内膜异位症女性基础及受刺激的PM合成细胞因子TNFα、IL-8和IL-10的水平高于正常。TNFα和IL-8水平与PF中的水平相关，提示PM是PF中这些细胞因子的主要来源。
