Li Q X, Camerini D, Xie Y, Greenwald M, Kuritzkes D R, Chen I S
Department of Microbiology, UCLA School of Medicine, Los Angeles, California, 90095, USA.
Virology. 1996 Apr 1;218(1):279-84. doi: 10.1006/viro.1996.0192.
Infection by human T-cell leukemia viruses (HTLV-I and HTLV-II) induces syncytium formation in certain cell types in vitro. Recombinant vaccinia viruses expressing the HTLV-II envelope (Env) gp61-II were used to infect human cells, and syncytium formation was observed, demonstrating that HTLV-II Env expression was sufficient to mediate syncytium formation in appropriate cells. This syncytium formation could be blocked by sera from HTLV-II-infected individuals. Infection with these recombinant vaccinia viruses caused fusion in mouse/human hybrid cells containing only human chromosome 17, whereas there was no obvious syncytium formation in mouse cells. This fusogenic phenotype of recombinant gp61-II is likely a property of the specific interaction between HTLV-II envelope protein and the HTLV cellular receptor, encoded by a gene that was previously indicated to be on human chromosome 17. On this basis, we developed a sensitive syncytium formation assay, using lacZ gene activation, for detecting the presence of the HTLV receptor(s). We used this fusion assay to test for HTLV receptor activity on a variety of cells. Our findings indicate that the HTLV receptor is widely distributed among species and cell types, including, to a limited extent, murine cells.
人类T细胞白血病病毒（HTLV-I和HTLV-II）感染在体外可诱导某些细胞类型形成多核巨细胞。用表达HTLV-II包膜（Env）糖蛋白gp61-II的重组痘苗病毒感染人类细胞，观察到多核巨细胞形成，表明HTLV-II Env表达足以介导合适细胞中的多核巨细胞形成。这种多核巨细胞形成可被HTLV-II感染个体的血清阻断。用这些重组痘苗病毒感染仅含人类17号染色体的小鼠/人类杂交细胞会导致融合，而在小鼠细胞中未观察到明显的多核巨细胞形成。重组gp61-II的这种融合表型可能是HTLV-II包膜蛋白与HTLV细胞受体之间特异性相互作用的特性，该受体由先前表明位于人类17号染色体上的一个基因编码。在此基础上，我们开发了一种利用lacZ基因激活的敏感多核巨细胞形成试验，用于检测HTLV受体的存在。我们用这种融合试验检测了多种细胞上的HTLV受体活性。我们的研究结果表明，HTLV受体广泛分布于不同物种和细胞类型中，包括在一定程度上的鼠类细胞。
