Schmalz G, Schweikl H, Esch J, Hiller K A
Clinic of Operative Dentistry and Periodontology, University of Regensburg, Germany.
J Endod. 1996 Mar;22(3):112-5. doi: 10.1016/s0099-2399(96)80285-8.
In this study, cell reaction after exposure to several dental cements was determined using a dentin barrier test device. The reaction of mouse fibroblasts grown on the "pulpal" side of a bovine dentin disk was determined after exposure to dental cements applied on the "cavity" side of the disk. For pretreatment, dentin disks (500-micrometer-thick) were etched on one side and either sterilized by autoclaving or disinfected with ethanol. It was found for both pretreatment groups that zinc phosphate cement was less toxic than the conventional glass ionomer cements. A light-cured glass ionomer cement and zinc oxide-eugenol were the most toxic materials tested. Disinfecting the dentin slices instead of autoclaving reduced the toxicity of the phosphate cement and the glass ionomer cements tendentiously. Because the strong cytotoxic reaction evoked by zinc oxide-eugenol and by the glass ionomer cements is in contrast to in vivo findings, further improvements of the dentin barrier test device will be necessary.
在本研究中，使用牙本质屏障测试装置测定了几种牙科粘固剂暴露后的细胞反应。在将牙科粘固剂施加于牛牙本质盘“洞腔”侧后，测定在牛牙本质盘“牙髓”侧生长的小鼠成纤维细胞的反应。对于预处理，将（500微米厚的）牙本质盘一侧蚀刻，然后通过高压灭菌消毒或用乙醇消毒。对于两个预处理组，均发现磷酸锌粘固剂的毒性低于传统玻璃离子粘固剂。一种光固化玻璃离子粘固剂和氧化锌丁香酚是所测试的毒性最大的材料。对牙本质切片进行消毒而非高压灭菌会有倾向性地降低磷酸粘固剂和玻璃离子粘固剂的毒性。由于氧化锌丁香酚和玻璃离子粘固剂引起的强烈细胞毒性反应与体内研究结果相反，因此有必要对牙本质屏障测试装置进行进一步改进。
