Santos P M, Williams S L, Thomas S S
Division of Otolaryngology, Southern Illinois University, Springfield 62794, USA.
J Neurosci Methods. 1995 Sep-Oct;61(1-2):79-84. doi: 10.1016/0165-0270(95)00026-q.
We have developed a rat gait analysis model to evaluate if ankle angle and other associated gait parameters could consistently define normal peroneal nerve and anterior tibialis muscle function. The second part of the study was designed to determine if such a model would be useful to measure recovery of function after a peroneal nerve crush injury (NCI). A clear plexiglas tunnel was designed for high-speed frame videotaping and subsequent computergraphic gait measurement and analysis. Normal gait patterns for ankle angle, back height, step and stride lengths and the stance and swing times were determined in 8 rats. Data analysis demonstrated no significant left/right differences for any of the variables (ANOVA) with the exception of step length. Subsequently, 12 rats with a peroneal NCI were evaluated. All gait parameters evaluated from the injured side were significantly different from the uninjured side after injury except stride length. Ankle angle was the most sensitive outcome variable. Weekly gait analysis provided objective measurements as the ankle angle gradually returned to normal within 3 weeks. The rat gait model is a sensitive and reproducible method for non-invasive evaluation of neuromuscular function during nerve recovery after a peroneal crush injury.
我们开发了一种大鼠步态分析模型，以评估踝关节角度和其他相关步态参数是否能够始终如一地界定正常的腓总神经和胫前肌功能。该研究的第二部分旨在确定这样一个模型对于测量腓总神经挤压伤（NCI）后功能恢复是否有用。设计了一个透明有机玻璃隧道用于高速逐帧录像以及随后的计算机图形步态测量与分析。测定了8只大鼠踝关节角度、背部高度、步长和步幅以及站立和摆动时间的正常步态模式。数据分析表明，除步长外，所有变量（方差分析）的左右差异均无统计学意义。随后，对12只患有腓总神经挤压伤的大鼠进行了评估。受伤后，除步幅外，从受伤侧评估的所有步态参数与未受伤侧均有显著差异。踝关节角度是最敏感的结果变量。每周进行的步态分析提供了客观测量数据，因为踝关节角度在3周内逐渐恢复正常。大鼠步态模型是一种用于在腓总神经挤压伤后神经恢复过程中对神经肌肉功能进行非侵入性评估的敏感且可重复的方法。
