Loubradou G, Bégueret J, Turcq B
Laboratoire de Génétique Moléculaire des Champignons Filamenteux, UPR CNRS 9026, Université de Bordeaux II, France.
Gene. 1996 Apr 17;170(1):119-23. doi: 10.1016/0378-1119(95)00847-0.
To identify cellular functions involved in vegetative incompatibility in filamentous fungi, we have initiated the cloning of Podospora anserina (Pa) mod genes. These genes interfere with the lethal reaction triggered by interaction between incompatible het genes. A gene (Pa AC) has been cloned by complementation of developmental defects caused by a mutation in the mod-D gene. This gene encodes a protein of 2145 amino acids (aa)that exhibits strong similarities with many adenylate cyclases (AC). About 65% aa identity has been found between the sequence of the polypeptide encoded by this Pa AC gene and the AC of Neurospora crassa. The organization of peptidic domains in the polypeptide encoded by Pa AC is closely related to that of Saccharomyces cerevisiae CYR1. Restriction-fragment-length polymorphism (RFLP) and genetic analysis have shown that Pa AC and mod-D are distinct genes.
为了确定丝状真菌营养体不相容性所涉及的细胞功能，我们已开始克隆嗜热栖热放线菌（Pa）的mod基因。这些基因会干扰由不相容的het基因之间相互作用引发的致死反应。通过互补mod-D基因突变引起的发育缺陷，克隆了一个基因（Pa AC）。该基因编码一种由2145个氨基酸（aa）组成的蛋白质，与许多腺苷酸环化酶（AC）具有很强的相似性。在该Pa AC基因编码的多肽序列与粗糙脉孢菌的AC之间发现了约65%的氨基酸同一性。Pa AC编码的多肽中的肽结构域组织与酿酒酵母CYR1密切相关。限制性片段长度多态性（RFLP）和遗传分析表明，Pa AC和mod-D是不同的基因。
