Deleu C, Clavé C, Bégueret J
Laboratoire de Génétique, Université de Bordeaux II, URA CNRS 542, Talence, France.
Genetics. 1993 Sep;135(1):45-52. doi: 10.1093/genetics/135.1.45.
Vegetative incompatibility is known to limit heterokaryosis in filamentous fungi. It results from genetic differences between incompatible strains at specific loci. The proteins encoded by the two incompatible alleles het-s and het-S of the fungus Podospora anserina differ from each other by 14 amino acids. Two approaches have been used to identify how many and which of these differences are necessary to elicit incompatibility. Twelve alleles of the het-s locus of wild-type isolates of P. anserina and of the related species Podospora comata have been sequenced to determine the extent of the variability of genes controlling s and S specificities. Expression of hybrid het-s/het-S genes and site-specific mutagenesis revealed that the specificities of het-s and het-S are under the control of a limited number of amino acid differences. The results show that vegetative incompatibility between s and S strains can be attributed to a single amino acid difference in the proteins encoded by the het-s locus.
已知营养体不亲和性会限制丝状真菌中的异核现象。它是由不亲和菌株在特定基因座上的遗传差异导致的。粪生粪壳菌（Podospora anserina）的两个不亲和等位基因het-s和het-S编码的蛋白质彼此相差14个氨基酸。已经采用了两种方法来确定这些差异中有多少以及哪些差异对于引发不亲和性是必需的。对粪生粪壳菌野生型分离株以及相关物种科马粪壳菌（Podospora comata）的het-s基因座的12个等位基因进行了测序，以确定控制s和S特异性的基因的变异程度。杂合het-s/het-S基因的表达和位点特异性诱变表明，het-s和het-S的特异性受有限数量的氨基酸差异控制。结果表明，s和S菌株之间的营养体不亲和性可归因于het-s基因座编码的蛋白质中的单个氨基酸差异。
