单个氨基酸差异足以引发真菌栗色柄锈菌中的营养体不亲和性。
A single amino acid difference is sufficient to elicit vegetative incompatibility in the fungus Podospora anserina.
作者信息
Deleu C, Clavé C, Bégueret J
机构信息
Laboratoire de Génétique, Université de Bordeaux II, URA CNRS 542, Talence, France.
出版信息
Genetics. 1993 Sep;135(1):45-52. doi: 10.1093/genetics/135.1.45.
Abstract
Vegetative incompatibility is known to limit heterokaryosis in filamentous fungi. It results from genetic differences between incompatible strains at specific loci. The proteins encoded by the two incompatible alleles het-s and het-S of the fungus Podospora anserina differ from each other by 14 amino acids. Two approaches have been used to identify how many and which of these differences are necessary to elicit incompatibility. Twelve alleles of the het-s locus of wild-type isolates of P. anserina and of the related species Podospora comata have been sequenced to determine the extent of the variability of genes controlling s and S specificities. Expression of hybrid het-s/het-S genes and site-specific mutagenesis revealed that the specificities of het-s and het-S are under the control of a limited number of amino acid differences. The results show that vegetative incompatibility between s and S strains can be attributed to a single amino acid difference in the proteins encoded by the het-s locus.
摘要
已知营养体不亲和性会限制丝状真菌中的异核现象。它是由不亲和菌株在特定基因座上的遗传差异导致的。粪生粪壳菌（Podospora anserina）的两个不亲和等位基因het-s和het-S编码的蛋白质彼此相差14个氨基酸。已经采用了两种方法来确定这些差异中有多少以及哪些差异对于引发不亲和性是必需的。对粪生粪壳菌野生型分离株以及相关物种科马粪壳菌（Podospora comata）的het-s基因座的12个等位基因进行了测序，以确定控制s和S特异性的基因的变异程度。杂合het-s/het-S基因的表达和位点特异性诱变表明，het-s和het-S的特异性受有限数量的氨基酸差异控制。结果表明，s和S菌株之间的营养体不亲和性可归因于het-s基因座编码的蛋白质中的单个氨基酸差异。
相似文献
1
A single amino acid difference is sufficient to elicit vegetative incompatibility in the fungus Podospora anserina.单个氨基酸差异足以引发真菌栗色柄锈菌中的营养体不亲和性。
Genetics. 1993 Sep;135(1):45-52. doi: 10.1093/genetics/135.1.45.
2
Sequence diversity and unusual variability at the het-c locus involved in vegetative incompatibility in the fungus Podospora anserina.在真菌粗糙脉孢菌中，参与营养体不亲和性的het-c位点的序列多样性和异常变异性。
Curr Genet. 1995 Apr;27(5):466-71. doi: 10.1007/BF00311217.
3
Inactivation of the Podospora anserina vegetative incompatibility locus het-c, whose product resembles a glycolipid transfer protein, drastically impairs ascospore production.嗜热栖热放线菌营养体不亲和位点het-c失活，其产物类似于糖脂转移蛋白，会严重损害子囊孢子的产生。
Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):5927-31. doi: 10.1073/pnas.91.13.5927.
4
A gene responsible for vegetative incompatibility in the fungus Podospora anserina encodes a protein with a GTP-binding motif and G beta homologous domain.在真菌粗糙脉孢菌中，一个负责营养体不亲和性的基因编码一种具有GTP结合基序和Gβ同源结构域的蛋白质。
Gene. 1995 Aug 30;162(1):135-9. doi: 10.1016/0378-1119(95)00272-8.
5
An additional copy of the adenylate cyclase-encoding gene relieves developmental defects produced by a mutation in a vegetative incompatibility-controlling gene in Podospora anserina.腺苷酸环化酶编码基因的额外拷贝可缓解由嗜热栖热放线菌中营养不亲和控制基因突变产生的发育缺陷。
Gene. 1996 Apr 17;170(1):119-23. doi: 10.1016/0378-1119(95)00847-0.
6
Natural variation of heterokaryon incompatibility gene het-c in Podospora anserina reveals diversifying selection.嗜热栖热放线菌中异核体不相容基因het-c的自然变异揭示了多样化选择。
Mol Biol Evol. 2014 Apr;31(4):962-74. doi: 10.1093/molbev/msu047. Epub 2014 Jan 20.
7
HET-E and HET-D belong to a new subfamily of WD40 proteins involved in vegetative incompatibility specificity in the fungus Podospora anserina.HET-E和HET-D属于WD40蛋白的一个新亚家族，该亚家族参与了粪生粪壳菌营养体不亲和特异性。
Genetics. 2002 May;161(1):71-81. doi: 10.1093/genetics/161.1.71.
8
The mod-A suppressor of nonallelic heterokaryon incompatibility in Podospora anserina encodes a proline-rich polypeptide involved in female organ formation.嗜鸟粪盘菌中非等位异核体不相容性的mod-A抑制因子编码一种参与雌器形成的富含脯氨酸的多肽。
Genetics. 1998 Jun;149(2):915-26. doi: 10.1093/genetics/149.2.915.
9
Mutational analysis of the [Het-s] prion analog of Podospora anserina. A short N-terminal peptide allows prion propagation.嗜热栖热放线菌[Het-s]朊病毒类似物的突变分析。一个短的N端肽段可实现朊病毒的传播。
Genetics. 1999 Dec;153(4):1629-40. doi: 10.1093/genetics/153.4.1629.
10
Identification of the het-r vegetative incompatibility gene of Podospora anserina as a member of the fast evolving HNWD gene family.将嗜热栖热放线菌的het-r营养体不亲和基因鉴定为快速进化的HNWD基因家族的成员。
Curr Genet. 2009 Feb;55(1):93-102. doi: 10.1007/s00294-008-0227-5. Epub 2009 Jan 10.
引用本文的文献
1
A Story Between s and S: [Het-s] Prion of the Fungus .s与S之间的故事：真菌的[Het-s]朊病毒
Mycobiology. 2024 Mar 9;52(2):85-91. doi: 10.1080/12298093.2024.2322211. eCollection 2024.
2
het-B allorecognition in Podospora anserina is determined by pseudo-allelic interaction of genes encoding a HET and lectin fold domain protein and a PII-like protein.在 Podospora anserina 中，het-B 同种异体识别由编码 HET 和凝集素折叠结构域蛋白以及 PII 样蛋白的假等位基因互作决定。
PLoS Genet. 2024 Feb 12;20(2):e1011114. doi: 10.1371/journal.pgen.1011114. eCollection 2024 Feb.
3
Emergence of the fungal immune system.真菌免疫系统的出现。
iScience. 2023 May 2;26(6):106793. doi: 10.1016/j.isci.2023.106793. eCollection 2023 Jun 16.
4
Allorecognition genes drive reproductive isolation in Podospora anserina.同种识别基因驱动 Podospora anserina 的生殖隔离。
Nat Ecol Evol. 2022 Jul;6(7):910-923. doi: 10.1038/s41559-022-01734-x. Epub 2022 May 12.
5
Balancing selection at nonself recognition loci in the chestnut blight fungus, Cryphonectria parasitica, demonstrated by trans-species polymorphisms, positive selection, and even allele frequencies.栗疫病菌非自身识别位点的平衡选择，通过跨物种多态性、正选择甚至等位基因频率来证明。
Heredity (Edinb). 2018 Dec;121(6):511-523. doi: 10.1038/s41437-018-0060-7. Epub 2018 Feb 10.
6
The HET-S/s Prion Motif in the Control of Programmed Cell Death.HET-S/s朊病毒基序在程序性细胞死亡调控中的作用
Cold Spring Harb Perspect Biol. 2016 Sep 1;8(9):a023515. doi: 10.1101/cshperspect.a023515.
7
As a toxin dies a prion comes to life: A tentative natural history of the [Het-s] prion.一种毒素消亡，一种朊病毒诞生：[Het-s] 朊病毒的初步自然史。
Prion. 2015;9(3):184-9. doi: 10.1080/19336896.2015.1038018.
8
Signal transduction by a fungal NOD-like receptor based on propagation of a prion amyloid fold.基于朊病毒淀粉样折叠传播的真菌NOD样受体信号转导。
PLoS Biol. 2015 Feb 11;13(2):e1002059. doi: 10.1371/journal.pbio.1002059. eCollection 2015 Feb.
9
Contribution of specific residues of the β-solenoid fold to HET-s prion function, amyloid structure and stability.β-螺旋折叠的特定残基对HET-s朊病毒功能、淀粉样结构和稳定性的贡献。
PLoS Pathog. 2014 Jun 12;10(6):e1004158. doi: 10.1371/journal.ppat.1004158. eCollection 2014 Jun.
10
The genome and development-dependent transcriptomes of Pyronema confluens: a window into fungal evolution.《共生真菌Pyronema confluens 的基因组和发育相关转录组：揭示真菌进化的窗口》
PLoS Genet. 2013;9(9):e1003820. doi: 10.1371/journal.pgen.1003820. Epub 2013 Sep 19.
本文引用的文献
1
[On the cytoplasmic factors associated with a pair of S-s genes in Podospora anserina].
C R Hebd Seances Acad Sci. 1959 Nov 30;249:2392-4.
3
Heterokaryon compatibility of unrelated strains in the Aspergillus nidulans group.构巢曲霉组中不相关菌株的异核体相容性
Heredity (Edinb). 1963 May;18:191-204. doi: 10.1038/hdy.1963.21.
4
DNAs of the two mating-type alleles of Neurospora crassa are highly dissimilar.粗糙脉孢菌两种交配型等位基因的DNA高度不同。
Science. 1988 Jul 29;241(4865):570-3. doi: 10.1126/science.2840740.
5
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.使用热稳定DNA聚合酶进行引物引导的DNA酶促扩增。
Science. 1988 Jan 29;239(4839):487-91. doi: 10.1126/science.2448875.
6
Single step large scale site-directed in vitro mutagenesis using multiple oligonucleotides.使用多个寡核苷酸的单步大规模定点体外诱变
Nucleic Acids Res. 1990 Dec 25;18(24):7457-8. doi: 10.1093/nar/18.24.7457.
7
Neurospora crassa A mating-type region.粗糙脉孢菌A交配型区域。
Proc Natl Acad Sci U S A. 1990 Jul;87(13):4912-6. doi: 10.1073/pnas.87.13.4912.
8
repa, a repetitive and dispersed DNA sequence of the filamentous fungus Podospora anserina.repa，一种丝状真菌嗜热栖热放线菌的重复且分散的DNA序列。 （注：原文中Podospora anserina翻译为嗜热栖热放线菌可能有误，一般翻译为“灰盖鬼伞”，这里按照你提供的英文准确翻译，但从生物学角度看该翻译可能与实际物种不符，你可根据实际情况调整。） 准确译文：repa，丝状真菌灰盖鬼伞的一种重复且分散的DNA序列。
Nucleic Acids Res. 1990 Aug 25;18(16):4901-3. doi: 10.1093/nar/18.16.4901.
9
Two allelic genes responsible for vegetative incompatibility in the fungus Podospora anserina are not essential for cell viability.负责食线虫孢菌营养体不亲和性的两个等位基因对细胞生存力并非必不可少。
Mol Gen Genet. 1991 Aug;228(1-2):265-9. doi: 10.1007/BF00282475.
10
Cloning the mating types of the heterothallic fungus Podospora anserina: developmental features of haploid transformants carrying both mating types.克隆异宗配合真菌嗜热栖热放线菌的交配型：携带两种交配型的单倍体转化体的发育特征
Genetics. 1991 Jul;128(3):539-47. doi: 10.1093/genetics/128.3.539.
Zotero 插件