Saupe S, Turcq B, Bégueret J
Laboratoire de Génétique, UPR CNRS 9026, Université de Bordeaux II, Talence, France.
Gene. 1995 Aug 30;162(1):135-9. doi: 10.1016/0378-1119(95)00272-8.
The het-e-1 gene of the fungus Podospora anserina is responsible for vegetative incompatibility through specific interactions with different alleles of the unlinked gene, het-c. Coexpression of two incompatible genes triggers a cell death reaction that prevents heterokaryon formation. The het-e1 allele has been cloned to get information on the function of the locus. It encodes a putative 1356-amino-acid polypeptide that displays two sequence motifs that have not yet been reported to be present on a single polypeptide. They are a GTP-binding domain and a repeated region that shares similarity with that of the beta-transducin. Contrary to other members of the beta-transducin family, sequence conservation between the repeated units is very strong and the number of repeats is different in wild-type het-e alleles.
真菌嗜鸟粪盘菌（Podospora anserina）的het - e - 1基因通过与不连锁基因het - c的不同等位基因发生特异性相互作用，导致营养体不亲和。两个不亲和基因的共表达引发细胞死亡反应，从而阻止异核体形成。het - e1等位基因已被克隆，以便获取该基因座功能的相关信息。它编码一个推定的1356个氨基酸的多肽，该多肽具有两个序列基序，尚未报道它们同时存在于单个多肽上。它们是一个GTP结合结构域和一个与β - 转导蛋白具有相似性的重复区域。与β - 转导蛋白家族的其他成员相反，重复单元之间的序列保守性非常强，并且在野生型het - e等位基因中重复次数不同。
