Franke C M, Leenhouts K J, Haandrikman A J, Kok J, Venema G, Venema K
Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan, Haren, The Netherlands.
J Bacteriol. 1996 Mar;178(6):1766-9. doi: 10.1128/jb.178.6.1766-1769.1996.
Four in-frame translational fusions to both the reporter proteins beta-galactosidase and alkaline phosphatase support a topological model of LcnD, a protein implicated in the transport of several bacteriocins from Lactococcus lactis, in which the N-terminal part is located intracellularly and one transmembrane helix spans the cytoplasmic membrane.
与报告蛋白β-半乳糖苷酶和碱性磷酸酶的四个读码框内翻译融合支持了LcnD的拓扑模型,LcnD是一种与乳酸乳球菌中几种细菌素的运输有关的蛋白质,其中N端部分位于细胞内,一个跨膜螺旋跨越细胞质膜。