Bujía J, Wilmes E, Kastenbauer E, Gürtler L
Department of Otorhinolaryngology, Ludwig Maximilians University, Munich, Germany.
Laryngoscope. 1996 May;106(5 Pt 1):645-7. doi: 10.1097/00005537-199605000-00024.
Since chemically preserved allogenic transplants have an established place in reconstructive procedures, the possibility of transferring the human immunodeficiency virus (HIV) with these transplants has been intensively discussed. In this study the authors obtained brain and spleen samples from six HIV-infected cadavers and preserved them with Merthiolate, Cialit, and formaldehyde. After preservation, the tissues were examined for proviral HIV-1 DNA (gag, pol, env) using the polymerase chain reaction. Proviral sequences were clearly demonstrated after the preservation procedure. The results of this study indicate that HIV remains in tissues that have been treated with Merthiolate, formaldehyde, or Cialit. Further investigations are necessary to determine if the virus is in an inactivated or activated form. It can be concluded that, because of the possible transmission of HIV by chemically preserved homografts, serologic screening of donors should be mandatory.
由于化学保存的同种异体移植在重建手术中已占有一席之地，因此对于通过这些移植传播人类免疫缺陷病毒（HIV）的可能性已进行了深入讨论。在本研究中，作者从6具感染HIV的尸体上获取了脑和脾样本，并用硫柳汞、西曲溴铵和甲醛进行保存。保存后，使用聚合酶链反应检测组织中的HIV-1前病毒DNA（gag、pol、env）。保存程序后明显检测到前病毒序列。本研究结果表明，HIV存在于经硫柳汞、甲醛或西曲溴铵处理过的组织中。有必要进一步研究以确定病毒是处于失活还是激活状态。可以得出结论，由于化学保存的同种异体移植可能传播HIV，因此对供体进行血清学筛查应成为强制性要求。
