Gomes E M, Souto P R, Felzenszwalb I
CETOX-Departamento de Biofísica e Biometria, Universidade do Estado do Rio de Janeiro, Brazil.
Mutat Res. 1996 Apr 6;367(4):204-8.
Natural products from flora and fauna are frequently used as nutritional supplements and medicaments. Two short-term assays were carried out and negative results were obtained for shark-cartilage containing preparation. The tests employed were the Salmonella/mammalian microsome assay using tester strains TA97, TA98, TA100, TA102 and TA1535 with or without S9 mix and the SOS-Chromotest with Escherichia coli strain PQ37. Evidence for shark-cartilage containing preparation functioning as an antimutagen was detected. Using bacterial survival assays with Escherichia coli fpg (BH20) and xthA (BW9091), we investigated the putative role of shark-cartilage containing preparation in protecting cells against lesions induced by hydrogen peroxide in normal and low iron level conditions. Our data suggest that shark-cartilage containing preparation can play a scavenger role for reactive oxygen species and protect against DNA lesions in both conditions.
来自动植物的天然产物常被用作营养补充剂和药物。进行了两项短期试验，含鲨鱼软骨制剂的试验结果为阴性。所采用的试验为使用测试菌株TA97、TA98、TA100、TA102和TA1535并添加或不添加S9混合物的沙门氏菌/哺乳动物微粒体试验，以及使用大肠杆菌菌株PQ37的SOS-显色试验。检测到含鲨鱼软骨制剂具有抗诱变作用的证据。通过对大肠杆菌fpg（BH20）和xthA（BW9091）进行细菌存活试验，我们研究了含鲨鱼软骨制剂在正常和低铁水平条件下保护细胞免受过氧化氢诱导损伤的假定作用。我们的数据表明，含鲨鱼软骨制剂在这两种条件下都可以对活性氧起到清除剂的作用，并防止DNA损伤。
