Yoshida T, Ikuta K, Sugaya H, Maki K, Takagi M, Kanazawa H, Sunaga S, Kinashi T, Yoshimura K, Miyazaki J, Takaki S, Takatsu K
Department of Immunology, Faculty of Medicine, University of Tokyo, Japan.
Immunity. 1996 May;4(5):483-94. doi: 10.1016/s1074-7613(00)80414-8.
We generated interleukin-5 receptor alpha chain (IL-5R alpha)-deficient (IL-5R alpha-/-) mice by gene targeting. The IL-5R alpha-/- mice showed decreased numbers of B-1 cells concomitant with low serum concentrations of IgM and IgG3. They showed no IL-5-induced enhancement of B cell responses to T-independent antigens. The number of alpha beta T cell receptor-positive thymocytes tended to decrease in 3-week-old IL-5R alpha-/- mice, returning to normal by 6 weeks of age. The IL-5R alpha-/- mice produced basal levels of eosinophils, while their bone marrow cells failed to form eosinophilic colonies in response to IL-5. Impaired eosinophilopoiesis in IL-5R alpha-/-mice enhanced the survival of Angiostrongylus cantonensis. These results indicate that IL-5-induced eosinophils serve as potent effector cells in the killing of Angiostrongylus cantonensis in mice.
我们通过基因打靶技术培育出白细胞介素-5受体α链（IL-5Rα）缺陷型（IL-5Rα-/-）小鼠。IL-5Rα-/-小鼠的B-1细胞数量减少，同时血清中IgM和IgG3浓度较低。它们未表现出IL-5诱导的B细胞对非依赖T细胞抗原反应的增强。在3周龄的IL-5Rα-/-小鼠中，αβT细胞受体阳性胸腺细胞数量有减少趋势，到6周龄时恢复正常。IL-5Rα-/-小鼠产生基础水平的嗜酸性粒细胞，而其骨髓细胞对IL-5无反应，无法形成嗜酸性粒细胞集落。IL-5Rα-/-小鼠中嗜酸性粒细胞生成受损，增强了广州管圆线虫的生存能力。这些结果表明，IL-5诱导的嗜酸性粒细胞在小鼠体内杀死广州管圆线虫的过程中作为有效的效应细胞发挥作用。
