通过在大肠杆菌中过表达磷酸烯醇丙酮酸羧化酶提高琥珀酸产量。
Enhanced production of succinic acid by overexpression of phosphoenolpyruvate carboxylase in Escherichia coli.
作者信息
Millard C S, Chao Y P, Liao J C, Donnelly M I
机构信息
Department of Chemical Engineering, Texas A&M University, College Station 77843, USA.
出版信息
Appl Environ Microbiol. 1996 May;62(5):1808-10. doi: 10.1128/aem.62.5.1808-1810.1996.
Abstract
Fermentative production of succinic acid from glucose by Escherichia coli was significantly increased by overexpression of phosphoenolpyruvate carboxylase. In contrast, overexpression of phosphoenolpyruvate carboxykinase had no effect. Under optimized conditions, induction of the carboxylase resulted in a 3.5-fold increase in the concentration of succinic acid, making succinic acid the major fermentation product by weight.
摘要
通过过表达磷酸烯醇丙酮酸羧化酶，大肠杆菌从葡萄糖发酵生产琥珀酸的产量显著增加。相比之下，过表达磷酸烯醇丙酮酸羧激酶则没有效果。在优化条件下，羧化酶的诱导使琥珀酸浓度增加了3.5倍，按重量计算，琥珀酸成为主要发酵产物。
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