van den Besselaar A M
Department of Haematology, Leiden University Hospital, The Netherlands.
Br J Haematol. 1996 May;93(2):437-44. doi: 10.1046/j.1365-2141.1996.5011041.x.
In a multicentre study of four thromboplastin reference materials (three from rabbit brain and one from bovine brain combined with adsorbed bovine plasma), three types of lyophilized plasmas were included. One was a pooled normal plasma, another was made by pooling plasmas of coumarin-treated patients, and the third was prepared by adsorption of vitamin K dependent clotting factors (artificially depleted). The purpose of this study was to investigate whether these lyophilized plasmas could be used for reliable calibration of the four thromboplastins. Calibration line slopes were calculated for the lyophilized plasmas and compared to the slopes based on fresh plasmas. When a rabbit thromboplastin was calibrated against a similar but not identical reagent (like-to-like comparison), the slopes for lyophilized plasmas and for fresh plasmas were the same. However, significant differences between lyophilized and fresh plasmas were observed in the calibration of unlike thromboplastins. The deviation of the slope with artificially depleted plasma was greater than that with lyophilized coumarin plasma. Indiscriminate use of these lyophilized plasmas for thromboplastin calibration should be avoided.
在一项对四种凝血活酶参考物质（三种来自兔脑，一种来自牛脑与吸附牛血浆的混合物）的多中心研究中，纳入了三种冻干血浆。一种是混合正常血浆，另一种是通过混合香豆素治疗患者的血浆制成，第三种是通过吸附维生素K依赖性凝血因子（人工耗尽）制备的。本研究的目的是调查这些冻干血浆是否可用于对这四种凝血活酶进行可靠校准。计算了冻干血浆的校准线斜率，并与基于新鲜血浆的斜率进行比较。当用一种类似但不完全相同的试剂对兔凝血活酶进行校准时（同类比较），冻干血浆和新鲜血浆的斜率相同。然而，在不同凝血活酶的校准中，观察到冻干血浆和新鲜血浆之间存在显著差异。人工耗尽血浆的斜率偏差大于冻干香豆素血浆的斜率偏差。应避免不加区分地使用这些冻干血浆进行凝血活酶校准。
