Poller L, van den Besselaar A M, Jespersen J, Tripodi A, Houghton D
Central Facility, ECAA, Department of Pathological Sciences, University of Manchester, UK.
Br J Haematol. 1998 Jun;101(3):462-7. doi: 10.1046/j.1365-2141.1998.00728.x.
Artificially-depleted lyophilized plasmas and lyophilized coumarin plasmas were prepared and compared with fresh coumarin plasmas to assess their comparative reliability in local thromboplastin calibration using the manual prothrombin time (PT) technique. Their certified PT values were inserted in turn on the vertical axis in place of the PT obtained with fresh coumarin plasmas. PT results were obtained at eight ECAA national laboratories ('test centres') and inserted on the horizontal axis. The resulting thromboplastin calibration slopes were compared with conventional fresh coumarin plasma calibration slopes at the same 'test centres'. When 60 artificially-depleted plasmas were substituted for 60 fresh plasmas, the mean calibration slopes with the human plain International Reference Preparation (IRP) were 4.2% higher. For comparison with 20 lyophilized coumarins, three sets of 20 artificially-depleted plasmas were selected in sequential order from the 60. The lyophilized coumarin plasmas gave a mean deviation of 9.6% from the fresh plasma calibration slopes compared with values of 2.0%, 6.1% and 11.7% for the three sets of 20 depleted plasmas. Although both types of lyophilized plasma calibration slopes give measurable differences from conventional fresh plasmas, these may be regarded as acceptable in clinical terms.
制备了人工耗尽冻干血浆和冻干香豆素血浆，并将其与新鲜香豆素血浆进行比较，以评估使用手工凝血酶原时间（PT）技术进行局部凝血活酶校准的相对可靠性。它们的认证PT值依次插入纵轴，以代替用新鲜香豆素血浆获得的PT值。在八个欧洲临床化学协会（ECAA）国家实验室（“测试中心”）获得PT结果，并插入横轴。将所得的凝血活酶校准斜率与相同“测试中心”的传统新鲜香豆素血浆校准斜率进行比较。当用60份人工耗尽血浆替代60份新鲜血浆时，使用人普通国际参考制剂（IRP）的平均校准斜率高4.2%。为了与20份冻干香豆素进行比较，从60份中依次选取三组各20份人工耗尽血浆。冻干香豆素血浆与新鲜血浆校准斜率的平均偏差为9.6%，而三组各20份耗尽血浆的值分别为2.0%、6.1%和11.7%。虽然两种类型的冻干血浆校准斜率与传统新鲜血浆相比都有可测量的差异，但从临床角度来看，这些差异可能被认为是可以接受的。
