Taylor-Papadimitriou J, Shearer M, Tilly R
J Natl Cancer Inst. 1977 Jun;58(6):1563-71. doi: 10.1093/jnci/58.6.1563.
Cells that can be cultured from pools of early-lactation milk were studied. Under the culture conditions used, the majority of cells attached to collagen-coated dishes; most of these remained single, did not divide, and in their adhesiveness, phagocytic ability, and ultrastructure resembled macrophages or histiocytes. On a plate seeded with approximately 3 X 10(5) cells, however, 10-100 colonies of dividing cells developed. These cells had the junctional complexes typical of epithelial cells and grew well in a medium supplemented with human serum and hydrocortisone for 16-20 days after seeding. After removal of serum from the medium, some cells continued to traverse the cell cycle, and the colonies containing these cells were morphologically distinct from those which became quiescent. The nondividing cells in milk could be separated from the milk epithelial cells and were able to stimulate the growth of epithelial cultures from benign mammary dysplasias.
对可从初乳池中培养的细胞进行了研究。在所使用的培养条件下，大多数细胞附着于胶原包被的培养皿；其中大多数细胞保持单个状态，不分裂，并且在黏附性、吞噬能力和超微结构方面类似于巨噬细胞或组织细胞。然而，在接种了约3×10⁵个细胞的平板上，出现了10 - 100个分裂细胞的集落。这些细胞具有上皮细胞典型的连接复合体，接种后在补充了人血清和氢化可的松的培养基中生长良好，持续16 - 20天。从培养基中去除血清后，一些细胞继续经历细胞周期，含有这些细胞的集落在形态上与那些进入静止状态的集落不同。乳汁中的非分裂细胞可以与乳腺上皮细胞分离，并且能够刺激来自良性乳腺发育异常的上皮细胞培养物的生长。
