Yomota C, Yoshii Y, Takahata T, Okada S
National Institute of Health Sciences, Osaka Branch, Japan.
J Chromatogr A. 1996 Jan 15;721(1):89-96. doi: 10.1016/0021-9673(95)00761-x.
The separation of human insulin (HI) and related compounds such as A-21 monodesamido HI (A-21DHI) and B-3 monodesamido HI (B-3DHI) was investigated using reversed-phase HPLC and capillary zone electrophoresis (CZE). In the case of HPLC under the usual acidic conditions, whereas A-21DHI was separately eluted, B-3DHI was included in the HI peak. Then it was found that by applying a novel ODS column resistant to alkaline conditions, a good separation of B-3DHI from HI could be achieved using an alkaline eluent, and two peaks corresponding to B-3DHI were eluted in front of the HI peak.
使用反相高效液相色谱法（HPLC）和毛细管区带电泳法（CZE）研究了人胰岛素（HI）与相关化合物如A-21单去酰胺基HI（A-21DHI）和B-3单去酰胺基HI（B-3DHI）的分离情况。在通常酸性条件下的HPLC分析中，A-21DHI能被单独洗脱，而B-3DHI则包含在HI峰中。随后发现，通过使用一种新型的耐碱性条件的ODS柱，采用碱性洗脱液可以实现B-3DHI与HI的良好分离，并且在HI峰之前洗脱出色谱图上对应B-3DHI的两个峰。
