Krajcovicová-Kudlácková M, Ozdín L
Research Institute of Nutrition, Bratislava, Slovak Republic.
Vet Med (Praha). 1995 Sep;40(9):293-8.
The effect of cadmium intake (100 micrograms/kg body weight/day as cadmium chloride over a period of three months) on the prooxidative-antioxidative state of liver was studied in 30 days old weaned male rats. Animals were fed a nutritionally balanced lacto-vegetable diet containing high-quality amino acid mixture (casein + gluten 1:1), lipids in form of either pork fat PF (% of polyunsaturated fatty acids PUFA = 11.9; unsaturation index UI = 72), margarine MA (% PUFA = 21.9; UI = 98), or soybean oil SO (% PUFA = 61.2; UI = 156) and vitamin E at amount of 60 mg/kg of food (groups PF, PF + Cd, MA, MA + Cd, SO, SO + Cd) or 600 mg (groups PF + Cd + E, MA + Cd + E, SO + Cd + E) in form of alpha-tocopheryl acetate. The following parameters were measured: conjugated dienes of fatty acids (CD), activities of superoxide dismutase (SOD), catalase (CAT) (as relative generation of H2O2) and glutathione peroxidase (GSH-Px). A direct relation between lipoperoxidation values and unsaturation index of lipids was found both in spontaneous (PF, MA, SO-control) and cadmium-induced generation of free oxygen radicals. Cadmium intake resulted in a disbalance in prooxidative-antioxidative processes which was manifested in a significant increase of CD in all fat sources (the degree of increase was directly proportional to UI and PUFA), in similar values of relative H2O2 generation and in a nonsignificant increase of GSH-Px in animals with most developed lipoperoxidation (SO). A tenfold increase in the administered dose of vitamin E restored a prooxidative-antioxidative equilibrium disturbed by cadmium intake in the liver of rats fed the diet with animal fat (PF + Cd + E) and margarine (MA + Cd + E) (reduction of CD to the level of control groups, decrease of relative generation of H2O2-significant in MA). In animals fed with soybean oil, a vitamin E-induced reduction of CD was significantly over the control level simultaneously with significant stimulation of GSH-Px activity. No changes in H2O2 generation together with CD levels and GSH-Px activity indicated that a synergic effect of several antioxidants is essential in the case of high lipoperoxidation. Presented results are important with respect to possible control or regulation of the equilibrium between prooxidative and antioxidative processes by nutrition.
在30日龄断奶雄性大鼠中研究了镉摄入（以氯化镉形式，每天100微克/千克体重，持续三个月）对肝脏氧化还原状态的影响。给动物喂食营养均衡的乳类 - 蔬菜饮食，其中包含优质氨基酸混合物（酪蛋白 + 谷蛋白1:1）、以下几种形式的脂质：猪肉脂肪PF（多不饱和脂肪酸PUFA含量 = 11.9%；不饱和度指数UI = 72）、人造黄油MA（PUFA含量 = 21.9%；UI = 98）或大豆油SO（PUFA含量 = 61.2%；UI = 156），以及每千克食物中含60毫克维生素E（PF、PF + Cd、MA、MA + Cd、SO、SO + Cd组）或以醋酸生育酚形式存在的600毫克维生素E（PF + Cd + E、MA + Cd + E、SO + Cd + E组）。测量了以下参数：脂肪酸共轭二烯（CD）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT，以H2O2的相对生成量表示）和谷胱甘肽过氧化物酶（GSH - Px）的活性。在自发的（PF、MA、SO - 对照组）和镉诱导的游离氧自由基生成过程中，均发现脂质过氧化值与脂质不饱和度指数之间存在直接关系。镉摄入导致氧化还原过程失衡，这表现为所有脂肪来源中CD显著增加（增加程度与UI和PUFA成正比）、相对H2O2生成量相似，以及在脂质过氧化最严重的动物（SO组）中GSH - Px无显著增加。在喂食动物脂肪（PF + Cd + E）和人造黄油（MA + Cd + E）的大鼠肝脏中，维生素E给药剂量增加十倍恢复了因镉摄入而扰乱的氧化还原平衡（CD降至对照组水平，MA组中相对H2O2生成量显著降低）。在喂食大豆油的动物中，维生素E诱导的CD降低显著超过对照组水平，同时GSH - Px活性显著受到刺激。H2O2生成量以及CD水平和GSH - Px活性均无变化，这表明在高脂质过氧化情况下，几种抗氧化剂的协同作用至关重要。就通过营养控制或调节氧化还原过程之间的平衡而言，所呈现的结果具有重要意义。
