对照及修复缺陷型人二倍体成纤维细胞1中DNA聚合酶α、β和γ的水平
Levels of DNA polymerases alpha, beta, and gamma in control and repair-deficient human diploid fibroblasts 1.
作者信息
Parker V P, Lieberman M W
出版信息
Nucleic Acids Res. 1977 Jun;4(6):2029-37. doi: 10.1093/nar/4.6.2029.
Abstract
The activities of DNA polymerases alpha, beta, and gamma were determined in control and repair-deficient human fibroblasts (xeroderma pigmentosum complementation groups A, C, and D; Fanconi's Anemia; and Bloom's syndrome). Assays were done on 103,000XG supernatants which had been chromatographed on DEAE cellulose to remove nucleic acids and on fractions containing polymerase activities which had been separated from one another on a second DEAE cellulose column. All repair-deficient cell types contained all three DNA polymerase activities. Caffeine, which has been observed to inhibit some DNA-repair processes in intact cells, had no effect on DNA polymerase activities from XP-A, XP-C, XP-D or XP-variant cells. These data indicate that all three polymerases are present in cells which have reduced or absent repair functions and that the caffeine effects observed in living cells are probably not due to the direct action of caffeine on DNA polymerases.
摘要
在对照及修复缺陷型人成纤维细胞（着色性干皮病互补组A、C和D；范可尼贫血；以及布卢姆综合征）中测定了DNA聚合酶α、β和γ的活性。检测是在103,000XG上清液上进行的，该上清液已在DEAE纤维素上进行色谱分离以去除核酸，并在含有已在第二个DEAE纤维素柱上彼此分离的聚合酶活性的级分上进行检测。所有修复缺陷型细胞类型均含有所有三种DNA聚合酶活性。咖啡因已被观察到可抑制完整细胞中的某些DNA修复过程，但对来自XP - A、XP - C、XP - D或XP变异细胞的DNA聚合酶活性没有影响。这些数据表明，所有三种聚合酶都存在于修复功能降低或缺失的细胞中，并且在活细胞中观察到的咖啡因效应可能并非由于咖啡因对DNA聚合酶的直接作用。
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