Tang Y, Othmer H G
Department of Mathematics, University of Utah, Salt Lake City 84112, USA.
Philos Trans R Soc Lond B Biol Sci. 1995 Aug 29;349(1328):179-95. doi: 10.1098/rstb.1995.0102.
In an earlier paper (Tang & Othmer 1994 Math. Biosci 120, 25-76), we developed a G-protein-based model for signal transduction in the cellular slime mould Dictyostelium discoideum and showed that it can account for the results from perfusion experiments done by Devreotes and coworkers (Devreotes et al. 1979 J. Cell. 80, 300-309; Devreotes & Steck 1979 J. Cell Biol. 80, 300-309; Dinauer et al. 1980 J. Cell Biol. 86, 537-561). The primary experimental observables are the amounts of cAMP secreted and the time scale of adaptation in response to various stimuli, and we showed that the predictions of the model agree well with the observations. Adaptation in the model arises from dual receptor-mediated pathways, one of which produces a stimulatory G protein Gs and the other of which produces an inhibitory G protein Gi. In this paper we use the model to simulate the suspension experiments of Gerisch & Wick (1975 Biochem. biophys. Res. Commun. 65, 364-370) and the experiments done in cell cultures on Petri dishes (Tomchik & Devreotes 1981 Science, Wash. 212, 443-446). The model predicts excitation to cAMP stimuli, sustained oscillations, or spiral waves and target patterns, depending on the developmental stage of the cells and experimental conditions. The interaction between different pacemakers is also studied.
在早期的一篇论文中（Tang和Othmer，1994年，《数学生物学》120卷，第25 - 76页），我们构建了一个基于G蛋白的细胞黏菌盘基网柄菌信号转导模型，并表明该模型能够解释Devreotes及其同事所做灌注实验的结果（Devreotes等人，1979年，《细胞杂志》80卷，第300 - 309页；Devreotes和Steck，1979年，《细胞生物学杂志》80卷，第300 - 309页；Dinauer等人，1980年，《细胞生物学杂志》86卷，第537 - 561页）。主要的实验可观测指标是分泌的cAMP量以及对各种刺激的适应时间尺度，并且我们表明该模型的预测与观测结果吻合得很好。模型中的适应源于双受体介导的途径，其中一条途径产生刺激性G蛋白Gs，另一条途径产生抑制性G蛋白Gi。在本文中，我们使用该模型来模拟Gerisch和Wick（1975年，《生物化学与生物物理学研究通讯》65卷，第364 - 370页）的悬浮实验以及在培养皿细胞培养中所做的实验（Tomchik和Devreotes，1981年，《科学》，华盛顿212卷，第443 - 446页）。该模型预测，根据细胞的发育阶段和实验条件，对cAMP刺激会产生兴奋、持续振荡或螺旋波以及靶形图案。我们还研究了不同起搏器之间的相互作用。
