Ackmann J J, Seitz M A, Dawson C A, Hause L L
Research Service, Department of Veterans Affairs Medical Center, Milwaukee, WI 53295, USA.
Ann Biomed Eng. 1996 Jan-Feb;24(1):58-66. doi: 10.1007/BF02770995.
The specific impedance of canine erythrocytes suspended in plasma was measured in the frequency range from 5 kHz to 1 MHz in samples from three animals in the hematocrit range from zero to packed cells at a temperature of 39 degrees C; measurements were made with a conductivity cell using four electrodes and a current density of 21 microA/cm2. With the use of impedance spectroscopy, data were fitted to an equivalent circuit model; model parameters in turn were fitted as functions of hematocrit. The resultant model can be used to predict specific impedance (real and reactive components) as a function of hematocrit and frequency over a frequency range from 5 kHz to 1 MHz and a hematocrit range from 0 to 80. Over a normal range of hematocrits and at frequencies less than 100 kHz, the current is almost exclusively confined to the plasma, and the specific impedance is nearly equal to the real component; however, at higher frequencies, the complex nature of specific impedance becomes important.
在39摄氏度下，对三只动物的样本进行测量，样本中血浆悬浮的犬红细胞比容从零到压实细胞，在5千赫兹至1兆赫兹的频率范围内测量其比阻抗；使用四电极电导池和21微安/平方厘米的电流密度进行测量。利用阻抗谱，将数据拟合到等效电路模型；模型参数又作为比容的函数进行拟合。所得模型可用于预测在5千赫兹至1兆赫兹的频率范围和0至80的比容范围内，比阻抗（实部和虚部）作为比容和频率的函数。在正常比容范围内且频率低于100千赫兹时，电流几乎完全局限于血浆中，比阻抗几乎等于实部；然而，在较高频率下，比阻抗的复数性质变得很重要。
