Karaki Y, Shimazaki K, Okamoto M, Ookami H, Fujimaki M
Second Department of Surgery, Toyama Medical and Pharmaceutical University, Japan.
Surg Today. 1996;26(1):5-11. doi: 10.1007/BF00311984.
We surgically prepared a hypergastrinemia model in rats and studied the effects of hypergastrinemia on chemically induced carcinogenesis in the esophagus. Operations were performed on 5-week-old male Donryu rats as follows: (1) truncal vagotomy plus pyloroplasty (group V), (2) segmental gastrectomy plus pyloroplasty (group G), (3) antrectomy (group A), and (4) no operation (group C) as a control. From the age of 6 weeks, the animals were given 0.003% N-methyl- N-amylnitrosamine (MAN) solution as drinking water for 8 weeks. After 20 weeks of MAN administration, the animals were bled and killed. The average serum gastrin levels in groups V and G were significantly higher than those groups C or A. There were significant differences between C and V in the incidence of carcinoma, and between V and A in the incidence of carcinoma including severe dysplasia. The incidence of histologically identified lesions per animal was determined, and significant differences were observed between C and both V and G in the incidence of carcinoma including severe dysplasia. Furthermore, we also detected gastrin receptors in the esophageal lesions produced by the oral administration of MAN to rats. The results of the present study suggest that endogenous hypergastrinemia has a positive influence on chemically induced carcinogenesis in the rat esophagus.
我们通过手术制备了大鼠高胃泌素血症模型，并研究了高胃泌素血症对化学诱导的大鼠食管癌发生的影响。对5周龄雄性唐利玉大鼠进行如下手术：（1）迷走神经干切断术加幽门成形术（V组）；（2）胃部分切除术加幽门成形术（G组）；（3）胃窦切除术（A组）；（4）不做手术（C组）作为对照。从6周龄开始，给动物饮用含0.003%N-甲基-N-戊基亚硝胺（MAN）的溶液，持续8周。给予MAN 20周后，对动物进行采血并处死。V组和G组的平均血清胃泌素水平显著高于C组或A组。C组和V组在癌发生率上存在显著差异，V组和A组在包括重度发育异常的癌发生率上存在显著差异。确定了每只动物经组织学鉴定的病变发生率，在包括重度发育异常的癌发生率方面，C组与V组和G组之间均观察到显著差异。此外，我们还在给大鼠口服MAN所产生的食管病变中检测到了胃泌素受体。本研究结果表明，内源性高胃泌素血症对化学诱导的大鼠食管癌发生有积极影响。
