Meseguer J, Esteban M A, Mulero V
Department of Cell Biology, Faculty of Biology, University of Murcia, Spain.
Anat Rec. 1996 Apr;244(4):499-505. doi: 10.1002/(SICI)1097-0185(199604)244:4<499::AID-AR8>3.0.CO;2-Q.
In higher vertebrates, cytolytic lymphocytes lyse their targets by the mechanisms of cell death called necrosis and apoptosis. However, the mechanisms of target cell death mediated by fish cytotoxic cells have not been established. We report the ultrastructure of target cell death mediated by the seawater teleosts gilthead seabream (Sparus aurata) and sea bass (Dicentrarchus labrax).
Head-kidney, blood, and peritoneal exudate leukocytes were incubated with HeLa or B16 melanoma tumor cells, as targets, and processed for transmission electron microscopic studies.
After incubation of tumor cells with leukocytes, substantial ultrastructural changes typically associated with both necrosis and apoptosis were observed in the plasmalemma, nucleus, and cytoplasm of the tumor cells. These morphological changes included loss of microvilli, the formation of long cytoplasmic processes and blebs, condensation and margination of the chromatin, swelling of the organelles, and vacuolation and condensation of the cytoplasm.
Our results indicate that leukocytes from gilthead seabream and sea bass are able to kill their targets by the mechanisms of necrosis and apoptosis, in a similar way to mammalian cytotoxic cells.
在高等脊椎动物中，细胞溶解性淋巴细胞通过坏死和凋亡这两种细胞死亡机制裂解其靶细胞。然而，鱼类细胞毒性细胞介导的靶细胞死亡机制尚未明确。我们报道了海水硬骨鱼金头鲷（Sparus aurata）和海鲈（Dicentrarchus labrax）介导的靶细胞死亡的超微结构。
将头肾、血液和腹腔渗出液白细胞与作为靶细胞的HeLa或B16黑色素瘤肿瘤细胞一起孵育，并进行透射电子显微镜研究。
肿瘤细胞与白细胞孵育后，在肿瘤细胞的质膜、细胞核和细胞质中观察到了通常与坏死和凋亡相关的大量超微结构变化。这些形态学变化包括微绒毛的丧失、长细胞质突起和泡的形成、染色质的凝聚和边缘化、细胞器的肿胀以及细胞质的空泡化和凝聚。
我们的结果表明，金头鲷和海鲈的白细胞能够通过坏死和凋亡机制杀死其靶细胞，这与哺乳动物细胞毒性细胞的方式相似。
