Pandey R, Quinn J, Joyner C, Murray D W, Triffitt J T, Athanasou N A
Nuffield Department of Orthopaedic Surgery, University of Oxford, United Kingdom.
Ann Rheum Dis. 1996 Jun;55(6):388-95. doi: 10.1136/ard.55.6.388.
To study the pathogenesis of aseptic loosening: in particular, to determine whether macrophages responding to particles of biomaterials commonly used in arthroplasty surgery for arthritis are capable of differentiating into osteoclastic bone resorbing cells, and the cellular and hormonal conditions required for this to occur.
Biomaterial particles (polymethylmethacrylate, high density polyethylene, titanium, chromium-cobalt, stainless steel) were implanted subcutaneously into mice. Macrophages were isolated from the foreign body granulomas that resulted, cultured on bone slices and coverslips, and assessed for both cytochemical and functional evidence of osteoclast differentiation.
Tartrate resistant acid phosphatase (TRAP) negative macrophages isolated from granulomas containing particles of all types of biomaterial composition were capable of differentiating into TRAP positive cells capable of extensive lacunar bone resorption (assessed by scanning electron microscopy). The presence of both UMR106 rat osteoblast-like cells and 1,25-dihydroxy vitamin D3 was necessary for this to occur.
All implant materials produce wear particles that are the focus of a heavy foreign body macrophage response in the fibrous membrane between a loose implant component and the host bone undergoing resorption. These findings underline the importance of biomaterial wear particle generation and the macrophage response to different types of biomaterial wear particles in the pathogenesis of aseptic loosening.
研究无菌性松动的发病机制，特别是确定对关节炎置换手术中常用生物材料颗粒产生反应的巨噬细胞是否能够分化为破骨细胞样骨吸收细胞，以及发生这种情况所需的细胞和激素条件。
将生物材料颗粒（聚甲基丙烯酸甲酯、高密度聚乙烯、钛、铬钴、不锈钢）皮下植入小鼠体内。从由此产生的异物肉芽肿中分离出巨噬细胞，在骨切片和盖玻片上培养，并评估破骨细胞分化的细胞化学和功能证据。
从含有所有类型生物材料成分颗粒的肉芽肿中分离出的抗酒石酸酸性磷酸酶（TRAP）阴性巨噬细胞能够分化为能够进行广泛陷窝骨吸收的TRAP阳性细胞（通过扫描电子显微镜评估）。UMR106大鼠成骨细胞样细胞和1,25-二羟基维生素D3的存在是发生这种情况的必要条件。
所有植入材料都会产生磨损颗粒，这些颗粒是松动植入部件与正在吸收的宿主骨之间纤维膜中强烈异物巨噬细胞反应的焦点。这些发现强调了生物材料磨损颗粒的产生以及巨噬细胞对不同类型生物材料磨损颗粒的反应在无菌性松动发病机制中的重要性。
