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粪肠球菌CRL 35产生的一种“类片球菌素”细菌素——肠球菌素CRL 35的纯化及N端氨基酸序列

Purification and N-terminal amino acid sequence of Enterocin CRL 35, a 'pediocin-like' bacteriocin produced by Enterococcus faecium CRL 35.

作者信息

Farías M E, Farías R N, de Ruiz Holgado A P, Sesma F

机构信息

Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biologicas (INSIBIO), Tucumán, Argentina.

出版信息

Lett Appl Microbiol. 1996 Jun;22(6):417-9. doi: 10.1111/j.1472-765x.1996.tb01193.x.

DOI:10.1111/j.1472-765x.1996.tb01193.x
PMID:8695065
Abstract

Enterocin CRL 35, a bacteriocin produced by Enterococcus faecium CRL 35 that inhibits food-borne pathogens, was purified by precipitation with (NH4)2SO4, gel filtration, ion exchange and reverse phase chromatography. The partial N-terminal amino acid sequence indicated a strong homology with other 'pediocin-like bacteriocins' previously described.

摘要

肠球菌素CRL 35是由屎肠球菌CRL 35产生的一种可抑制食源性病原体的细菌素，通过硫酸铵沉淀、凝胶过滤、离子交换和反相色谱法进行纯化。其部分N端氨基酸序列显示与先前描述的其他“类片球菌素细菌素”有很强的同源性。

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