Kay E W, Mulcahy H E, Curran B, O'Donoghue D P, Leader M
Department of Pathology, Royal College of Surgeons in Ireland, Dublin.
Eur J Cancer. 1996 Apr;32A(4):612-6. doi: 10.1016/0959-8049(95)00596-x.
The DNA content of 168 consecutive T3,N0,M0 (Dukes' B, Astler-Coller B2) colorectal cancers was studied using image analysis on formalin-fixed paraffin-embedded tissues. 72 cases (43%) were classified as diploid and the remaining 96 (57%) as non-diploid. After a median follow-up period of 6.7 years, a significant survival advantage was found for diploid compared with non-diploid cases (logrank test; P = 0.008). The long-term (8 year) survival rate was 70% for diploid and 46% for non-diploid tumours. Subgroup analysis showed that the survival advantage conferred by tumour diploidy was greatest in large (> or = 5 cm) cancers and was found both in colonic and rectal cancer cases. These data indicate that tumour ploidy status measured by image analysis might be useful in determining risk of colorectal cancer recurrence and death in patients following resection of early colorectal cancer.
采用图像分析法，对168例连续的T3、N0、M0（杜克B期、阿斯特勒-科勒B2期）结肠直肠癌福尔马林固定石蜡包埋组织的DNA含量进行了研究。72例（43%）被归类为二倍体，其余96例（57%）为非二倍体。中位随访期为6.7年，结果发现二倍体病例的生存率显著高于非二倍体病例（对数秩检验；P = 0.008）。二倍体肿瘤的长期（8年）生存率为70%，非二倍体肿瘤为46%。亚组分析显示，肿瘤二倍体所带来的生存优势在大（≥5 cm）癌症中最为明显，且在结肠癌和直肠癌病例中均有发现。这些数据表明，通过图像分析测量的肿瘤倍体状态可能有助于确定早期结肠直肠癌切除术后患者的癌症复发和死亡风险。
