Lindmark G
Department of Medical and Physiological Chemistry, University of Uppsala, Sweden.
Br J Cancer. 1996 Nov;74(9):1413-8. doi: 10.1038/bjc.1996.557.
This study aimed to investigate whether immunohistochemical staining for nm23-H1 protein in the primary tumour is correlated with tumour stage, tumour differentiation, DNA ploidy, cell proliferative index, p53 status and patient survival time in colorectal cancer. Full-cross colorectal cancer biopsies were collected from 202 consecutive surgical specimens between 1987 and 1990. Immunohistochemical expression of nm23-H1 protein was investigated in cryosections, using a monoclonal anti-nm23-H1 antibody (clone NM 301). The staining pattern was classified as follows: strong homogeneous intensity, moderate homogeneous intensity, moderate focal intensity, or as negative. Immunohistochemical expression of p53 was investigated using a monoclonal anti-p53 antibody (DO-7). The DNA ploidy and cell proliferative index were determined by flow cytometry. Possible correlation between nm23-H1 staining patterns and the other studied tumour characteristics was explored at the end of 1994. Median survival time of living patients was 66 months, range 50-93 months. No correlation was found between various nm23-H1 staining patterns and tumour stage, cell proliferative index or p53 status. Nm23-H1-negative tumours and tumours with moderate focal staining intensity were less differentiated than tumours with strong homogeneous or moderate homogeneous staining intensity (P < 0.05). Of the nm23-H1-negative tumours, a significantly higher number was near-diploid rather than aneuploid, as compared with those expressing positive nm23-H1 (P < 0.05). The number of dead patients in Dukes' stages B and C did not correlate significantly with the nm23-H1 staining pattern. The nm23-H1 staining pattern alone, or combined with either of the other explored tumour characteristics, did not correlate with patient survival time. Immunohistochemical studies of the nm23-H1 protein expression are of minor value in the staging and prognostic prediction of colorectal cancer.
本研究旨在探讨原发性肿瘤中nm23-H1蛋白的免疫组化染色是否与结直肠癌的肿瘤分期、肿瘤分化、DNA倍体、细胞增殖指数、p53状态及患者生存时间相关。1987年至1990年间，从202例连续手术标本中收集全层结直肠癌活检组织。使用单克隆抗nm23-H1抗体（克隆NM 301），在冰冻切片中研究nm23-H1蛋白的免疫组化表达。染色模式分类如下：强均匀强度、中等均匀强度、中等局灶强度或阴性。使用单克隆抗p53抗体（DO-7）研究p53的免疫组化表达。通过流式细胞术测定DNA倍体和细胞增殖指数。1994年底探讨了nm23-H1染色模式与其他研究的肿瘤特征之间可能的相关性。存活患者的中位生存时间为66个月，范围为50 - 93个月。未发现各种nm23-H1染色模式与肿瘤分期、细胞增殖指数或p53状态之间存在相关性。与具有强均匀或中等均匀染色强度的肿瘤相比，nm23-H1阴性肿瘤和具有中等局灶染色强度的肿瘤分化程度较低（P < 0.05）。与表达阳性nm23-H1的肿瘤相比，nm23-H1阴性肿瘤中近二倍体的数量明显高于非整倍体（P < 0.05）。Dukes B期和C期死亡患者的数量与nm23-H1染色模式无显著相关性。单独的nm23-H1染色模式，或与其他探索的肿瘤特征之一相结合，均与患者生存时间无关。nm23-H1蛋白表达的免疫组化研究在结直肠癌的分期和预后预测中价值不大。
