大鼠肝脏100000g上清液对胆汁酸的结合作用。
Binding of bile acids by 100 000g supernatants from rat liver.
作者信息
Strange R C, Nimmo I A, Percy-Robb I W
出版信息
Biochem J. 1977 Mar 15;162(3):659-64. doi: 10.1042/bj1620659.
Abstract
- The binding of glycocholic acid, chenodeoxycholic acid and lithocholic acid to rat liver 1000 000g supernatants was studied by equilibrium dialysis. 2. The binding characteristics of the bile acids suggest that the binding components are involved in bile acid transport. 3. When mixtures of [14C]lithocholic acid and liver supernatants were eluted from columns of Sephadex G-75, a prominent peak of [14C]lithocholic acid appeared with proteins of mol.wt. approx. 40000. A second, smaller, peak of [14C]lithocholic acid was eluted with proteins of mol.wt. approx. 100000. 4. The inclusion of cholic acid, glycocholic acid or chenodeoxycholic acid in the eluting buffer decreased the amount of [14C]lithocholic acid that was eluted with the higher-molecular-weight component.
摘要
- 通过平衡透析研究了甘氨胆酸、鹅去氧胆酸和石胆酸与大鼠肝脏1000 000g上清液的结合情况。2. 胆汁酸的结合特性表明，结合成分参与胆汁酸的转运。3. 当[14C]石胆酸与肝脏上清液的混合物从Sephadex G - 75柱上洗脱时，[14C]石胆酸的一个突出峰出现在分子量约为40000的蛋白质处。[14C]石胆酸的第二个较小峰与分子量约为100000的蛋白质一起被洗脱。4. 在洗脱缓冲液中加入胆酸、甘氨胆酸或鹅去氧胆酸，会减少与高分子量成分一起洗脱的[14C]石胆酸的量。
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