胆碱能突触前神经末梢上的一种ATP激活的配体门控离子通道。
An ATP-activated, ligand-gated ion channel on a cholinergic presynaptic nerve terminal.
作者信息
Sun X P, Stanley E F
机构信息
Synaptic Mechanisms Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
出版信息
Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):1859-63. doi: 10.1073/pnas.93.5.1859.
Abstract
ATP has recently been identified as a fast neurotransmitter in both the central and peripheral nervous systems. Several studies have suggested that ATP can also affect the release of classical neurotransmitters, including acetylcholine with which it is co-released. We have searched for ATP receptors on a cholinergic presynaptic nerve terminal using the calyx-type synapse of the chicken ciliary ganglion. ATP was pulsed onto the terminals under voltage clamp and induced a short latency cation current that exhibited inward rectification and marked desensitization. This current was not seen with adenosine but was mimicked by several sterically restricted ATP analogs and was blocked by suramin. ATP-activated single ion channels exhibited prominent flickering and had a conductance of approximately 17 pS. Our results demonstrate a ligand-gated P2X-like purinergic receptor on a cholinergic presynaptic nerve terminal.
摘要
三磷酸腺苷（ATP）最近被确定为中枢和外周神经系统中的一种快速神经递质。多项研究表明，ATP还可影响经典神经递质的释放，包括与其共同释放的乙酰胆碱。我们利用鸡睫状神经节的花萼型突触，在胆碱能突触前神经末梢上寻找ATP受体。在电压钳制下，将ATP脉冲施加到神经末梢上，可诱导出一种潜伏期短的阳离子电流，该电流表现出内向整流和明显的脱敏现象。腺苷不会产生这种电流，但几种空间位阻受限的ATP类似物可模拟该电流，且该电流可被苏拉明阻断。ATP激活的单离子通道表现出明显的闪烁现象，电导约为17皮西门子。我们的结果证明，在胆碱能突触前神经末梢上存在一种配体门控的P2X样嘌呤能受体。
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