Weynants V, Tibor A, Denoel P A, Saegerman C, Godfroid J, Thiange P, Letesson J J
Unité d'Immunologie-Microbiologie, Universitaires Notre-Dame de la Paix, Namur, Belgium.
Vet Microbiol. 1996 Jan;48(1-2):101-12. doi: 10.1016/0378-1135(95)00153-0.
During the last four years, an increasing number of cattle herds were classified positive by brucellosis screening tests in areas of Belgium and France free of the disease. No clinical symptom of brucellosis was reported in these animals and no Brucella abortus strains were isolated. After two years, no brucellosis outbreak was registered in all of the herds concerned. On this basis, all the serological reactions observed were classified as false positive. An ELISA using Yersinia Outer membrane Proteins (YOPs) as antigens was developed in order to discriminate between a Yersinia enterocolitica O:9 infection and a Brucella abortus infection. Antibodies against YOPs were detected in sera from Y. enterocolitica O:9 experimentally infected cattle (n = 4) but not in sera from B. abortus experimentally infected cattle (n = 4). In a field study, 66.7% of the 174 serum samples from cattle presenting false positive serological reactions showed anti-YOPs antibodies whereas only 10% of 454 sera, classified negative by the brucellosis screening tests, showed anti-YOPs antibodies. Our results suggest that infections with Y. enterocolitica O:9 may cause false positive reactions in brucellosis testing.
在过去四年中，比利时和法国无布鲁氏菌病地区越来越多的牛群在布鲁氏菌病筛查试验中被判定为阳性。这些动物未报告布鲁氏菌病的临床症状，也未分离出流产布鲁氏菌菌株。两年后，所有相关牛群均未出现布鲁氏菌病疫情。在此基础上，所有观察到的血清学反应均被归类为假阳性。开发了一种以耶尔森氏菌外膜蛋白（YOPs）为抗原的酶联免疫吸附测定（ELISA），以区分小肠结肠炎耶尔森氏菌O:9感染和流产布鲁氏菌感染。在实验感染小肠结肠炎耶尔森氏菌O:9的牛（n = 4）血清中检测到了抗YOPs抗体，但在实验感染流产布鲁氏菌的牛（n = 4）血清中未检测到。在一项现场研究中，174份出现血清学反应假阳性的牛血清样本中有66.7%显示出抗YOPs抗体，而在454份经布鲁氏菌病筛查试验判定为阴性的血清中，只有10%显示出抗YOPs抗体。我们的结果表明，小肠结肠炎耶尔森氏菌O:9感染可能在布鲁氏菌病检测中导致假阳性反应。
