Kittelberger R, Reichel M P, Joyce M A, Staak C
Central Animal Health Laboratory, Wallaceville Animal Research Center, Upper Hunt, New Zealand.
Vet Microbiol. 1997 Oct 16;57(4):361-71. doi: 10.1016/s0378-1135(97)00110-7.
The course of immunological reaction in 10 Yersinia enterocolitica 0:9 experimentally-infected heifers was followed using the conventional brucellosis tests complement fixation test (CFT), serum agglutination test (SAT) and brucella card test (BCT), and a recently developed Brucella antigen-specific gamma interferon (IFN-gamma) test. Initially, the animals were exposed orally to 10(10) colony-forming units (CFU) of Y. enterocolitica 0:9. Four weeks later, they were inoculated intravenously with 10(8) CFU of Y. enterocolitica 0:9 cells. After oral inoculation, the response in the conventional brucellosis tests was minimal. Only after intravenous inoculation were CFT and SAT titres and BCT reactions comparable to natural, false positive brucellosis reactors. After oral exposure the Brucellergen-stimulated release of IFN-gamma peaked at values above the cut-off stimulation index of 2.5 in 80% of the heifers. After intravenous inoculation, stimulation indices above 2.5 were present in only 10% of the animals. Two B. abortus infected control cattle showed stimulation indices of 3.1 and 3.4, and a negative control animal exhibited a stimulation index of 1.0. These findings show, in contrast to a previous study, that the Brucellergen-specific IFN-gamma assay cannot be used as a specific and discriminatory test for B. abortus infections.
采用传统布鲁氏菌病检测方法（补体结合试验（CFT）、血清凝集试验（SAT）和布鲁氏菌卡片试验（BCT））以及最近开发的布鲁氏菌抗原特异性γ干扰素（IFN-γ）检测方法，跟踪10头经实验感染小肠结肠炎耶尔森菌0:9的小母牛的免疫反应过程。最初，给这些动物经口接种10¹⁰集落形成单位（CFU）的小肠结肠炎耶尔森菌0:9。四周后，给它们静脉注射10⁸CFU的小肠结肠炎耶尔森菌0:9细胞。经口接种后，传统布鲁氏菌病检测的反应极小。仅在静脉接种后，CFT和SAT滴度以及BCT反应才与自然感染、假阳性布鲁氏菌病反应动物相当。经口接触后，布鲁氏菌抗原刺激的IFN-γ释放量在80%的小母牛中达到高于2.5的临界刺激指数值的峰值。静脉接种后，只有10%的动物刺激指数高于2.5。两头感染流产布鲁氏菌的对照牛刺激指数分别为3.1和3.4，一头阴性对照动物刺激指数为1.0。与之前的一项研究相反，这些发现表明，布鲁氏菌抗原特异性IFN-γ检测不能用作流产布鲁氏菌感染的特异性和鉴别性检测。
